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《Hunan Journal of Traditional Chinese Medicine》 2015-12
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Establishment of a human colorectal cancer xenograft model on the chick embryo chorioallantoic membraned

YANG Xiao;JIANG Yi-lan;JIAN Xiao-lan;LI Yong-min;Hunan University of Chinese Medicine;The Affiliated Hospital of Hunan Academy of Chinese Medicine;  
Objective: To establish a human colorectal cancer xenograft model on the chick embryo chorioallantoic membrane( CAM). Methods: The chick embryo CAM model was made using fertilized chicken eggs incubated for 7days. Those eggs were randomly divided into five groups. Four groups received inoculation of 2. 5 × 106,5. 0 ×106,1. 0× 107,and 2. 0 × 107HCT116 cells in the avascular zones of gas chamber,respectively,while the control group received inoculation of an equal volume of PBS buffer. The survival and tumor development in th ose chicken embryos were evaluated after 7 days of incubation. Results: There was no significant difference in the survival between different groups of chicken embryos. The three groups inoculated with 5. 0 × 106,1. 0 × 107,and 2. 0 × 107HCT116 cells had the highest rates of tumor development. There was no significant difference in the rate of tumor development between the three groups( P 0. 05). Conclusion: The HCT116 cell xenograft tumors can develop on the chick embryo CAM. The optimal number of cells for inoculation is 5. 0 × 106.
【Fund】: 湖南省科技计划科研条件创新专项(编号:2013TT1004);; 湖南省研究生科研创新项目(编号:CX2015B329)
【CateGory Index】: R273;R-332
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