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《Hunan Journal of Traditional Chinese Medicine》 2015-12
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Establishment of a human colorectal cancer xenograft model on the chick embryo chorioallantoic membraned

YANG Xiao;JIANG Yi-lan;JIAN Xiao-lan;LI Yong-min;Hunan University of Chinese Medicine;The Affiliated Hospital of Hunan Academy of Chinese Medicine;  
Objective: To establish a human colorectal cancer xenograft model on the chick embryo chorioallantoic membrane( CAM). Methods: The chick embryo CAM model was made using fertilized chicken eggs incubated for 7days. Those eggs were randomly divided into five groups. Four groups received inoculation of 2. 5 × 106,5. 0 ×106,1. 0× 107,and 2. 0 × 107HCT116 cells in the avascular zones of gas chamber,respectively,while the control group received inoculation of an equal volume of PBS buffer. The survival and tumor development in th ose chicken embryos were evaluated after 7 days of incubation. Results: There was no significant difference in the survival between different groups of chicken embryos. The three groups inoculated with 5. 0 × 106,1. 0 × 107,and 2. 0 × 107HCT116 cells had the highest rates of tumor development. There was no significant difference in the rate of tumor development between the three groups( P 0. 05). Conclusion: The HCT116 cell xenograft tumors can develop on the chick embryo CAM. The optimal number of cells for inoculation is 5. 0 × 106.
【Fund】: 湖南省科技计划科研条件创新专项(编号:2013TT1004);; 湖南省研究生科研创新项目(编号:CX2015B329)
【CateGory Index】: R273;R-332
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Wang Qian1,Xu Yundan1,Zhao Gang1,2*(1.College of Basic Medicine,Hubei University of Chinese Medicine,Wuhan 430065,China; 2.Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Hubei University of TCM,Ministry of Education,Wuhan 430065,China);A Simple Method of Human Colon Carcinoma HCT-116 Cells Subculture[J];Asia-Pacific Traditional Medicine;2013-02
2 YANG Qi;BAI Yan-xia;YAN Li-ying;WANG Quan-ying;YANG Guang-xiao;Department of Otolaryngology-Head-Neck Surgery,the First Affiliated Hospital,Medical School of Xi′an Jiaotong University;Xi′an Huaguang Biotechnology Company;;Establishment of a transplantation tumor model of human anaplastic thyroid carcinoma in chick embryo and its biological characteristics[J];Journal of Xi'an Jiaotong University(Medical Sciences);2014-02
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 WANG Qian1,ZHAO Gang1,2(1.College of preclinical Medicine,Hubei University of Chinese Medicine,Wuhan 430065;2.Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription,Hubei University of Chinese Medicine,Ministry of Education,Wuhan 430065);Effect of Curcumin on Growth of Human Colon Carcinoma Cell Line HCT-116[J];Journal of Hubei University of Chinese Medicine;2012-01
2 CHEN Ke-li,JIANG Heng,CHEN Jian-fang,PEI Li,LIANG Hou-jie. Department of Oncology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China;Stem cell-like property of colon cancer cell line HCT116[J];Medical Journal of Chinese People's Liberation Army;2009-11
3 WANG Guo-qiang , FANG Yu-jing, LU Zhen-hai, PAN Zhi-zhong, WAN De-sen. Department of Gastrointestinal Surgery, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China;Effects of indomethacin on the proliferation and invasion of colon cancer cell line HCT116 and its mechanisms[J];The Journal of Practical Medicine;2012-11
4 ZHANG Bin1,RUAN Xi-yun2,CHEN Jie1,ZHANG Shi-bao1,LIU Shuang-qing1, WANG Quan-ying3,YANG Guang-xiao3,LIU Qing-yong1(1.Department of Urology,Jinan Central Hospital Affiliated to Shangdong University,Jinan 250013; 2.Department of Neurology,Shandong Provincial Hospital,Jinan 250021; 3.Xi'an Huaguang Biological Engineering Co.,Ltd.,Xi'an 710025,China);Establishment and biological characteristics of a human renal cell carcinoma model on chick embryo chorioallantoic membrane[J];Journal of Xi'an Jiaotong University(Medical Sciences);2012-01
5 Ling Zhang, Department of Disease Prevention and Health Protection, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China Lian Guo, Yong Peng, Bing Chen, Department of Endocrinology, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China;Effects of T-STAR gene on activity of telomerase in colon cancer cell line HCT-116[J];World Chinese Journal of Digestology;2005-11
6 LIN Lei1, YE Meng2, WANG Shao-ming2, NI Shu-min2, WU Xiao2 1Department of Dermatology, 2Department of Oncology, Affiliated Hospital of Medical College of Ningbo University, Ningbo 315020, Zhejiang, China;Mistletoe induces apoptosis by down-regulating the constitutive activation of nuclear factor-κB in HCT116 cells[J];Chinese Journal of Clinical Pharmacology and Therapeutics;2008-07
7 LI Xiao-ying,GAO Kai,LIU Xue-li,ZHANG Lian-feng(Key Laboratory of Human Diseases Comparative Medicine,Ministry of Health;Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences(CAMS) & Comparative Medicine Centre,Peking Union Medical Collage(PUMC),Beijing 100021,China);The Establishment of Human Colorectal Cancer Model Labeled by GFP and DsRed[J];Chinese Journal of Comparative Medicine;2009-09
8 WT6BX]SUN Hui, WANG Jin-guo,ZHANG De-heng, et al. Department of General Surgery, The Third Affiliated Hospital, Jilin University, Changchun 130031 [WT5”HZ];[Orthotopic Implantation Model for Human Anaplastic Thyroid Cancer Cell Line[J];Chinese Journal of Bases and Clincs In General Surgery;2002-05
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