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《Hunan Journal of Traditional Chinese Medicine》 2015-12
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Determination of the content of gypenosides in diet tea by ultraviolet spectrophotometry

LI Wei;Yueyang Second People's Hospital;  
Objective: To establish a method for determination of the content of gypenosides in diet tea by ultraviolet( UV) spectrophotometry. Methods: The absorbance of the developed color was read at 547 nm using a Shimadzu UV- 2450 spectrophotometer. Standard gypenosides were used as a control. The content of gypenosides was calculated by plotting a standard curve. Results: A good linear relationship with a regression equation of Y = 0. 00272X- 0. 04087 was achieved when the concentration of gypenosides ranged between 9. 19 and 45. 94μg / m L( r =0. 9993). The recovery averaged 88. 32%,and the relative standard deviation was 1. 9%. Conclusion: The established method in this study is fast,simple,precise,and reliable for determination of the content of gypenosides in diet tea without too many controls.
【CateGory Index】: R286.0;O657.3
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