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《Hunan Journal of Traditional Chinese Medicine》 2016-01
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Effects of icariin on ERβ gene expression and serum estradiol level in ovariectomized rats

LIU Shan;LI Jin-ping;YANG Lin;WANG Wen-jie;YANG Yan-bing;JING Xiu-ping;Hunan University of Chinese Medicine;School of Pharmaceutical Sciences,Central South University;  
Objective:To investigate the effects of icariin on serum estradiol(E_2) level and estrogen receptor β(ERβ) gene expression in bone tissue in ovariectomized rats.Methods:A total of 72 female Sprague- Dawley(SD)rats were selected and ovariectomized to establish the pathological model of osteoporosis,and randomly divided into high- dose icariin group(group A),low- dose icariin group(group B) group with a medium dose of bone- strengthening and pain- relieving prescription(group C),model group(group D),and estradiol valerate control group(group E);another 12 female SD rats were selected as the sham- operation group(group F);all the drugs were administered by gavage for 13 weeks.Eight rats were randomly selected in each group,and blood samples were collected from the abdominal aorta and were centrifuged after coagulation.The enzyme- linked immunosorbent assay was applied to measure serum E_2 level;mRNA samples were collected from femoral tissue and polymerase chain reaction was performed to measure ERβ gene expression.Results:All the administration groups showed a significant increase in serum E_2 level and a significant reduction in ERβ gene expression,and compared with group D groups A,C,E,and F had a significant increase in serum E_2 level and significantly upregulated expression of ERβ gene(P 0.05,P 0.01);the indicators above showed a significant difference between group B and group F(P 0.05,P 0.01).Conclusion:By improving serum E_2 level in ovariectomized rats,upregulating the mRNA expression of ERβ in bone tissue,and increasing the synthesis of ERβ,icariin can improve the biological effect of ER and achieve the anti- postmenopausal osteoporosis effect.
【Fund】: 国家自然科学基金资助项目(编号:81273816)
【CateGory Index】: R285.5
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