Molecular regulation mechanism of antiangiogenic effect of Tripterygium wilfordii compound TW3
WANG Li;LIU Yu-juan;ZHENG Yun-qiu;Medical College,Hexi University;Theorem Clinical Research( Shanghai) Co.,Ltd.;
Objective: To investigate the effect of various doses of TW3,a compound from Tripterygium wilfordii,on the mRNA expression of vascular endothelial growth factor( VEGF) and cyclooxygenase-2( COX-2) in human umbilical vein endothelial cells( HUVECs). Methods: The neonatal umbilical cord was used to isolate and culture the primary HUVEC cell line,single cell suspension was prepared after subculture,and the cultured cells were inoculated. The adherent cells were randomly divided into model group( phorbol ester was added),low-,medium-,and high-dose TW3 groups( 0. 01,0. 1,and 1μg/ml of TW3 was added,respectively),positive control group( phorbol ester and 5-fluorouracil injection were added),and blank group( no treatment). All the groups were incubated in the incubator with 5% CO_2 at a temperature of 37°C for 24 hours,and then RNA was extracted. Reverse transcription-polymerase chain reaction was used to measure the changes in the mRNA expression of VEGF and COX-2,and Smart View image processing software was applied for the quantification of optical density of electrophoretic band. Results: In all TW3 groups,TW3 significantly inhibited the mRNA expression of VEGF and COX-2( P 0. 05),and there was a dose-effect relationship. Conclusion: The molecular regulation mechanism of antiangiogenic effect of TW3 may be related to the down-regulated mRNA expression of VEGF and COX-2,and down-regulation is enhanced with increasing dose of TW3.
【CateGory Index】： R285