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Role of GF-β1/Smad signaling pathway in renal tissues in rats with renal fibrosis caused by adenine

CUI Zheng;MA Xiao-yan;Liaoning University of Traditional Chinese Medicine;The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine;  
Objective: To investigate the changes and role of TGF- β1 / Smad signaling pathway in the model of renal fibrosis induced by adenine. Methods: A total of 23 Sprague- Dawley rats were randoml y divided into normal group( 10 rats) and model group( 13 rats),and adenine was used to establish a rat model of renal fibrosis.RT- PCR was performed to measure the mRNA expression of tumor growth factor- β1( TGF- β1) in rats,ELISA was performed to measure the protein expression of Smad2,Smad3,and Smad7,and immunohistochemistry was performed to measure the protein expression of a- SMA. Results: Compared with the normal group,the model group showed significantly upregulated mRNA expression of TGF- β1,significant increases in the levels of Smad2 and Smad3,and a significant reduction in Smad7 level( P 0. 01). Conclusion: Renal fibrosis induced by adenine in rats is closely associated with the TGF- β1 / Smad signaling pathway,and the regulation of TGF- β1 / Smad signaling pathway plays an important role in inhibiting the development and progression of renal fibrosis.
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