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HPLC method for determining naringin in Ketanning mixture

WU Jin-song;LI Qin-wen;YUAN Qing-zhao;CHEN Ling-long;The Affiliated Hospital of Hunan Academy of Chinese Medicine;  
Objective: To investigate the high- performance liquid chromatography( HPLC) method for determining naringin in Ketanning mixture. Methods: HPLC was performed on a Welch Materials XB- C18column(4. 6mm ×150mm,5μm) with a mobile phase of methanol- 0. 8% acetic acid solution( 36: 64) at a wavelength of 283 nm and a flow rate of 1. 0m L / min. Results: Naringin showed a good linear relationship between concentration and peak area within the range of 1. 01- 202μg / m L( r = 0. 9999),and the average recovery rate was 96. 5%( RSD = 0. 4%).Conclusion: The HPLC method is simple,time- saving,and accurate and can be used for content determination and quality control of this preparation.
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