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《Journal of Huaqiao University(Natural Science)》 2012-01
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Highly Selective Determination of Uric Acid in Human Serum by Molecularly Imprinted Electrochemical Sensor

YIN Jing-fen,LIAN Hui-ting,SUN Xiang-ying,LIU Bin(College of Material Science and Engineering,Huaqiao University,Xiamen 361021,China)  
A highly selective molecularly imprinted electrochemical sensor of uric acid(UA) was prepared with chitosan(CS) as functional matrix and uric acid as template molecule via constant potential electrochemical deposition.The imprinted membrane was characterized by Attenuated total reflection infrared spectroscopy(ATR-FTIR) and Electrochemical impedance spectroscopy(EIS);the performances of the sensor were studied by cyclic voltammetry(CV) and differential pulse voltammetry(DPV).The UA had good electrochemical response in 0.1 mol·L-1 phosphate buffer solution(PBS)(pH=5.0).The oxidation peak current of DPV was well-proportional to the concentration of UA in the range from 0.1 μmol·L-1 to 80.0 μmol·L-1,with a correlation coefficient of 0.999 1.The developed sensor exhibited specific recognition for UA against the competitors which consisted of similar structure and coexisting interference in human serum.Moreover,the sensor also shows exellent reproducibility and was successfully employed to the determination of UA in human serum with the recovery of 97.41%~102.8%.
【Fund】: 国家自然科学基金资助项目(20955001 21175049);; 福建省自然科学基金计划资助项目(D0710017 D0810016 2011J01049);; 教育部留学回国人员科研启动基金资助项目(2008年度);; 国务院侨办科研基金资助项目(10QZR13)
【CateGory Index】: TP212.2
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