Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Huaqiao University(Natural Science)》 2012-01
Add to Favorite Get Latest Update

Highly Selective Determination of Uric Acid in Human Serum by Molecularly Imprinted Electrochemical Sensor

YIN Jing-fen,LIAN Hui-ting,SUN Xiang-ying,LIU Bin(College of Material Science and Engineering,Huaqiao University,Xiamen 361021,China)  
A highly selective molecularly imprinted electrochemical sensor of uric acid(UA) was prepared with chitosan(CS) as functional matrix and uric acid as template molecule via constant potential electrochemical deposition.The imprinted membrane was characterized by Attenuated total reflection infrared spectroscopy(ATR-FTIR) and Electrochemical impedance spectroscopy(EIS);the performances of the sensor were studied by cyclic voltammetry(CV) and differential pulse voltammetry(DPV).The UA had good electrochemical response in 0.1 mol·L-1 phosphate buffer solution(PBS)(pH=5.0).The oxidation peak current of DPV was well-proportional to the concentration of UA in the range from 0.1 μmol·L-1 to 80.0 μmol·L-1,with a correlation coefficient of 0.999 1.The developed sensor exhibited specific recognition for UA against the competitors which consisted of similar structure and coexisting interference in human serum.Moreover,the sensor also shows exellent reproducibility and was successfully employed to the determination of UA in human serum with the recovery of 97.41%~102.8%.
【Fund】: 国家自然科学基金资助项目(20955001 21175049);; 福建省自然科学基金计划资助项目(D0710017 D0810016 2011J01049);; 教育部留学回国人员科研启动基金资助项目(2008年度);; 国务院侨办科研基金资助项目(10QZR13)
【CateGory Index】: TP212.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 WANG Chang-qin~1,XU Hai-hong~2,HAN Xiao-gang~1 and WU Shou-guo~(*1)(1.Department of Chemistry,University of Science and Technology of China,Hefei 230026;2.Chemical Examination Section,The Third People′s Hospital of Hefei,Hefei 230022);Determination of uric acid in whole blood by using an activated glassy carbon electrode[J];Chinese Journal of Analysis Laboratory;2007-01
2 LIAN Hui-ting,CHEN Juan-juan,XUE Yan,LIU Bin(College of Material Science and Engineering,Huaqiao University,Quanzhou 362021,China);Electrochemcial Characteristic of Monocrotophos Molecularly Imprinted Sensor[J];Journal of Huaqiao University(Natural Science);2011-02
3 SUN Wei-dong~1,WANG Jin-yong~2,YE Cheng-rong~1,WANG Xiao-long~1(1.Inst of Nutr and Metab Dis in Anim and Fowls,Nanjing Agric Univ,Nanjing,210095,China;2.Coll of Agric,Hebei Univ of Engin,Handan,057150,China);Study on analysis of animal urinary stones by FTIR[J];Journal of Yandzhou University;2006-02
Chinese Journal Full-text Database 10 Hits
1 WU Gang,SHEN Yu-Hua,XIE An-Jian,LIN Hong-Yun(College of Chemistry and Chemical Engineering, Anhui University, Heifei230039,China);Studies on the adsorption of bilirubin in solution by chitosan[J];Journal of Anhui University(Natural Sciences);2003-01
2 SI You-lin1,2,ZHANG Sheng-yi1,CHEN Xiao-jing1,FANG Chun-xia1(1.School of Chemistry and Chemical Engineering,Anhui University,Hefei 230039,China;2.Department of Basic Courses,Bengbu Medical College,Bengbu 233000,China);Preparation of TiO_2 nanoparticles by using chitosan as template and their photocatalytic activity[J];Journal of Anhui University(Natural Sciences);2008-01
3 GAO Gui-zhen,LIU Xiao-yang(Biochemistry Analysis Laboratory,Suzhou College,Suzhou 234000,China);Study on the interaction between alizarin red S and chitosan by fluorescence method[J];Journal of Anhui University(Natural Sciences Edition);2010-04
4 Chen Jinjin(Department of Environmental Science,College of Environmental Science and Engineering,Zhong Shan University,Guangzhou,510275);Development of the Research of Chitin/Chitosan and Its Derivatives in Environmental Fields[J];Anhui Chemical Industry;2003-03
5 DING Chun-mei, SONG Qing-ping, ZHANG Ze-zheng (Dept. of Bioch. Engn., Anhui University of Technology and Science, Wuhu 2410 00, China);Orthogonal method of extracting protein from shrimp shell[J];Journal of Anhui Institute of Mechanical and Electrical Engineering;2003-01
6 LI Jian,BI Song-mei(Anhui Key Laboratory of Textile Materials,Anhui University of Technology and Science,Wuhu 241000,China);Research of intrinsic viscosity deviation of chitosan analyzing by one-point method[J];Journal of Anhui University of Technology and Science(Natural Science);2009-04
7 Wang Weiping Du Yumin Li Yan Shi Zhengyi;Study on the Production of Chitosan from Absidia coerulea with Cheap Raw Materials[J];Anhui Agricultural Science Bulletin;2007-20
8 ZHU Hua-yue et al (Department of Environmental Engineering,Taizhou University,Linhai,Zhejiang 317000);Study on the Adsorptiona and Removal of Protonated Cross-linked Chitosan Granules to Nitrate in Water Body[J];Journal of Anhui Agricultural Sciences;2008-07
9 LIU Shu-qi et al (School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237);Effects of Chitosan Coated Slow Release Fertilizers on the Crop Growth[J];Journal of Anhui Agricultural Sciences;2008-20
10 CAO Jia-lie et al(School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237);Study on Nutrient Slow Release Characteristics of Chitosan Coated Slow Release Fertilizers(CCF)[J];Journal of Anhui Agricultural Sciences;2008-23
China Proceedings of conference Full-text Database 10 Hits
1 ZHAN Yi-zhen ZHAO Xue WANG Wei 1 Saintyear Holding Group Co., Ltd, Hangzhou 311221, China 2 Chemical and Bioengineering college,Donghua University,Shanghai 200051, China;Manufacturing and Application of Antibacterial Alginate/Gelatin Blend Fiber[A];[C];2008
2 Liu xiaohua Li shumei Xia fanglian (Department of preventive medicine,Gannan medical university.Ganzhou,341400);The Progress on Chitosan and Chitooligosaccharides to Diabetes Mellitus[A];[C];2007
3 CHANG Jing LIU Wan-shun HAN Bao-qin LIU Bing (College of Marine Life Science,Ocean University of China,Qingdao 266003,China);Study and comparison of biological properties of carboxymethyl-chitosan and carboxymethyl-chitin[A];[C];2007
4 YU Min-na, WEI Chang-ping, L Zhi-jun, JIA Kun (School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, China);Preparation and performance of low-molecular weight N, O-carboxymethyl-chitosan[A];[C];2009
5 Zhou Yan-bin~1 Zeng Qing-xiao~2 Wu Xiao-yong~2 Ning Chu-guang~2 (1.Guangzhou Shuangqiao Company Ltd.,Guangzhou 510280) (2.College of Light Industry & Food Engineering,South China Univ.of Tech.,Guangzhou 510640);Chitosan and its Functions on Intestinal Health[A];[C];2004
6 GU Yue-xia,JIAO Qiang,LI Tao,SUI Wei-ping (School of Chemistry and Chemical Engineering,University of Jinan,Jinan 250022,China);Study on adsorption equilibrium and kinetics of chromium(Ⅵ) on zeolite loads chitosan adsorbents[A];[C];2009
7 Tao Xiqin~(1,2),~*Wang Mingli~1,Yuan Zhi~1,Wang Lijuan~2 (1.Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy,Guiyang,Guizhou 550003,China; 2.College of Chemical Engineering,Guizhou University,Guiyang,Guizhou 550003,China);Application and Properties Research of Nano-TiO_2-Chitosan Composite Films[A];Chinese Society of Agricultural Engineering[C];2009
8 Cao Weixing, Liu Ronghou, Sun Chen (Biomass Energy Engineering Research Centre, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240,China);Study on the absorption of sweet-stem sorghum juice with chitosan[A];Chinese Society of Agricultural Engineering[C];2009
9 Zhu LONG, Lina YU, Rong ZHANG (Key Laboratory of Science and Technology of Eco-Textile Ministry of Education, Jiangnan University, Wuxi 214122);Preparation of Amphoteric Chitosan and its application[A];[C];2007
10 ZHANG Rong,LONG Zhu (Key Laboratory of Science and Technology of Eco - Textile Ministry of Education,Southern Yangtze University,Wuxi 214122,china);The Preparation and application of Graft Copolymer of 2,3-epoxypropyltrimethylammonium Chloride onto Chitosan[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Shu-juan1,TAN Zheng-chu1,ZHONG Xing-gang1,WU Zai-sheng2,SHEN Guo-li21.Hunan Tea Research Institute,Hunan Academy of Agricultural Science,Changsha 410125,China;2.Chemical and Chemistry Institute,Hunan University,Changsha 410082,China;Studies on a Novel Parathion-Methyl Biosensor Design Based on Acetylcholinesterase Immobilized by Zirconia Nanoparticle[J];Chinese Journal of Sensors and Actuators;2009-05
2 CHEN Fan, CHEN Huan-lin, HE Yi (Coll of Materials Sci & Chem Engin, Zhejiang University, Hangzhou 310027,China);Advances in organophosphorus hydrolase biosensors and theirs applications[J];Journal of Transducer Technology;2004-04
3 Li Ping, Wu Shouguo *, Zhang Hanchang, Ma Chaoxiong (Department of Chemistry, University of Science and Technology of China, Hefei 230026);Electrochemical Behavior of Uric Acid on Prussian Blue Modified Electrode and Its Application to the Analytical Chemistry[J];Chinese Journal of Analytical Chemistry;2005-01
4 LIU Zhi-Hang, HUAN Shuang-Yan, SHEN Guo-Li~*, YU Ru-Qin (State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China);Detection of Cinchonine Using Molecularly Imprinted Electrosynthesized Polymer as Biomimetic Receptor Layers[J];Chemical Research In Chinese Universities;2005-06
5 Liu Bin Sun Xiangying Xu Jinrui (College of Mater. Sci. & Eng., Huaqiao Univ., 362011, Quanzhou, China);Application of Chitosan to Analytical Chemistry[J];Journal of Huaqiao University(Natural Science);2003-03
6 YANG Yun,LIAN Hui-ting,LIU Bin,SUN Xiang-ying(College of Material Science and Engineering,Huaqiao University,Quanzhou 362021,China);Preparation and Properties of Trichlorfon Molecularly Imprinted Polymers[J];Journal of Huaqiao University(Natural Science);2009-03
7 Shi Ruixue\+* Guo Chenghai Zou Xiaohong Zhu Chunye Zuo Yanjun Deng Yundu (Research Institute of Chemical Defence, Beijing 102205, China);The Development of Research in Molecular Imprinting Technique[J];Progress In Chemistry;2002-03
8 MA Yong-fei,QU Xiang-jin,AI Shi-yun,YIN Huan-shun,DU Hong-xia(College of chemistry and material science,Shandong Agricultural University,Taian 271018,China);Determination of atrazine in soil sample based on molecularly imprinted film electrochemical sensors[J];Chemical Research and Application;2009-05
9 ZHOU Gu-zhen,SUN Yuan-xi (Department of Chemistry,Hunan University of Arts and Science,Changde415000,China);Use of Poly(Crystal Violet)Film Modified Electrode to Measure Uric Acid[J];Journal of Instrumental Analysis;2004-06
10 Zhang Hanchang; Zuo Xiaobing; Ji Mingrong; Wu Shouguo(Department of Applied Chemistry Center of Structure and Element Analysis, University of Science and Technology of China, He Fei 230026);Properties of Anodically Oxidative Glass Carbon Electrodes (GCE) in Strong Alkaline Solutions[J];Acta Physico-chimica Sinica;1996-07
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved