Research on the Effect of the Intensity and Amount of Light Source to Maize Haploid Identification Based on Near Infrared Diffuse Transmission
QIN Hong;MA Jing-yi;CHEN Shao-jiang;LI Wei-jun;YAN Yan-lu;WANG Ping;LIU Jin;Institute of Semiconductors, Chinese Academy of Sciences;College of Information and Control Engineering, China University of Petroleum(Huadong);National Maize Improvement Center, China Agricultural University;College of Information and Electrical Engineering, China Agricultural University;
Aiming at the same batches of the same variety corn seeds, this paper studied the influence factors about the spectral curve quality and the recognition rate of maize haploid identification based on NIR qualitative analysis technology. The factors were studied from the two aspects which were the light intensity and diaphragm aperture with the diffuse transmission spectrum measurement way. Partial least squares-discriminant analysis(PLS-OLDA) was used to compress the pretreated spectral data, and then the identification models were built based on Support Vector Machine(SVM). The measured data were recorded under different experimental conditions and the recognition correct rates were calculated. The results showed that the signal to noise ratio of light spectral curve is affected by the amount of light through the diaphragm,and the closer to the seed size the aperture diameter of the diaphragm is, the smoother spectrum would be got.The intensity of light source directly influenced the spectrum curve's effect, and the stronger the light energy,the greater wave peak to valley difference, and at the same time the recognition rate is higher. The results showed that the recognition rate of maize haploid and hybrid reached 100% based on the higher light power and appropriate diaphragm.