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《West China Journal of Stomatology》 2014-01
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Preparation of sodium alginate-nanohydroxyapatite composite material for bone repair and its biocompatibility

Wang Yanmei;He Jiacai;Li Quanli;Shen Jijia;Dept. of Oral and Maxillofacial Surgery, Stomatological Hospital Affiliated to Anhui Medical University;Key Discipline of Clinical Science of Stomatology in Anhui Province,Province Laboratory of Oral Diseases, Central and Local Working Together for the Oral Medical Center Laboratory;Dept. of Microbiology and Parasitology, Anhui Medical University;  
Objective To prepare sodium alginate-nanohydroxyapatite composite material and to explore its feasibility as a bone repair material. Methods Sodium alginate-nanohydroxyapatite composite material was prepared using chemical cross-linking and freeze-drying technology. The composite was characterized by X-ray diffraction(XRD) and scanning electron microscope(SEM) and its porosity was measured by liquid displacement method. The fifth passage of bone marrow stromal stem cells(BMSCs) were incubated on the composite material and then growth was observed by inverted microscope and SEM. BMSCs were cultured with liquid extracts of the material, methyl thiazolyl tetrazolium(MTT) assay was used to calculate the relative growth rate(RGR) on 1, 3, 5 d and to evaluate the cytotoxicity. Fresh dog blood was added into the liquid extracts to conduct hemolysis test, the spectrophotometer was used to determine the optical density(OD) and to calculate the hemolysis rate. Results Sodium alginate-nanohydroxyapatite composite material displayed porosity, the porous pore rate was(88.6±4.5)%. BMSCs showed full stretching and vigorous growth under inverted microscope and SEM. BMSCs cultured with liquid extracts of the material had good activities. The toxicity of composite material was graded as 1. Hemolysis test results showed that the hemolysis rate of the composite material was 1.28%, thus meeting the requirement of medical biomaterials. Conclusion The composite material fabricated in this study has high porosity and good biocompatibility.
【Fund】: 安徽省自然科学基金资助项目(11040606M173);; 安徽医科大学博士科研启动基金资助项目(XJ201034)
【CateGory Index】: R318.08
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