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《Acta Chimica Sinica》 1998-01
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Hydrolytic Cleavage of Adenosine- 3'- monophosphate and Guanosine - 3' - monophosphate by Lanthanides

ZHU Bing LI Xin-Min WU Yi-Jie ZHAO Da-Qing NI Jia-Zuan (Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, The Chinese Academy of Science, Changchun, 130022)  
The hydrolytic cleavage of adenosine - 3' - monophosphate(3' - AMP) and guanosine -3' - monophosphate (3' - GMP) by lanthanides was investigated by NMR,HPLC and the method of measuring the liberated inorganic phosphate. The results show that lanthanides have specificity for hydrolyzing 3' - mononucleotides. 3' - AMP and 3' - GMP were converted to Adenosine(A),phosphate and Guanosine(G),phosphate respectively at pH 9,37℃ . The efficiency of cleavage was greater than that of 5'- mononucleotides. The mechanism of hydrolytic of cleavage was discussed in detail.
【Fund】: 国家自然科学基金;; 中国科学院基础局资助项目(29631010)
【CateGory Index】: O629
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