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《Biological Disaster Science》 2018-04
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Study on Fermentation Optimization of Antifungal Active Substance of Streptomyces corchorusii AUH-1

YANG Yong;ZHANG Yong;LI Shu-qin;ZHANG Shuai-wen;LIU Qun;LI Kun-tai;School of Bioscience and Bioengineering, Jiangxi Agricultural University;  
Streptomyces corchorusii AUH-1 had broad-spectrum in suppressing the growth of various fungal plant pathogens, such as Rhizoctonia solani and Fusarium oxysporum f.sp.niveum. In order to improve the inhibitory effect of S. corchorusii AUH-1 on R.solani, the response surface methodology was used to optimize the carbon source and nitrogen source in the culture medium in this paper. A single factor experiment was designed and tested with different concentration of carbon source and nitrogen source. According to the test results, the carbon source and nitrogen source were selected as variables. Design-Expert 8.05 b Software was used in the design of the test group of Box-Benhnken, the data were analyzed by using Design-Expert 8.05 b software, and the optimal combination concentration of carbon source and nitrogen source was obtained. The results showed that the optimal component level of each factor was 3.1% sucrose, 0.25% corn starch, 4.5% corn steep liquor and 0.375%ammonia sulfate, and the inhibitory effect of S. corchorusii AUH-1 on R.solani was the best under this concentration. The model prediction value was 70.5041% and the actual optimized value was 69.31%, which was5.78% higher than that of the basic medium. This study laid a foundation for the further development of rice sheath blight bio-control agents and fungicides.
【Fund】: 国家自然科学基金项目(209005220);; 江西省(青年)自然科学基金重大项目(20143ACB2100);; 江西省青年科学家培养计划项目(20142BCB23025)
【CateGory Index】: S476
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