A mouse line that express Cre recombinase by gene knock-in strategy
DONG Ming, YU Peng, RAO Ming li, et al (Department of Neurology, The First Hospital, Ji Lin University, Changchun 130021,China)
Objective To generate a neuron specific mouse line that expresses Cre recombinase and to study the gene functions in a specific tissue and temporal period in vivo using conditional gene knock out.Methods Using the knock in skill to express Cre recombinase under the control of murine microtubule associate protein 1a( Map1a) gene promoter. Results One of the mouse lines expressed the Cre recombinase by PCR. Conclusion It will be a new tool for studying functions of genes during the late phase of brain nerve cells development using conditional gene knock out strategy.
【CateGory Index】： Q784