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《Journal of Harbin Medical University》 2007-04
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Experimental investigation on construction of tissue-engineered osteo-musculo-cutaneous flaps

XU Xue-wu,GUO Tie-fang,HAN Xue-feng,et al (Department of Orthopaedics,The Second Clinical College,Harbin Medical University,Harbin 150081,China)  
Objective To construct a tissue-engineering muscle pedicel bone graft by culture marrow cells combined with biodegradable polymeric scoffolds(HA/TCP) which be used to repairing a segmental of long bone.Methods From December 2004 to January 2006,bone marrow stromal cells(BMSC) being aspirated from spina iliaca posterior superior of young rabbits were induced into osteoblasts in vitro.Osteoblasts which were isolated from bone marrow stromal cells of New Zealand rabbits were cultured as the seeding cells,and the cells were cultured with HA/TCP in virto.The complex was observed under phase microscope and histology to evaluate the interaction between osteoblasts and HA/ TCP.The compound co-cultured for 14 days was implanted intermuscularly into muscle latissimus dorsi of rabbits which were investigated at 6 weeks after postoperatively.The muscle pedicel bone graft were investigated by means of gross observation,histology of HE.Results Scanning electronic micrographs showed that the pores of HA/TCP and its surface were filled with cultured osteoblasts,especially in the longer cultured block,no cell growth was found in the central area,new bone formation was observed in all blocks at 6 weeks after implantation.Conclusion The osteoblasts from bone marrow stromal cells can be used as the seeded cells for bone tissue engineering,bio-derived materials being constituted with BMSC may be a good option in construction of bone tissue engineering.
【CateGory Index】: R318.1
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【Citations】
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