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《Journal of Harbin Medical University》 2012-01
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Screening and analyzing of the phage-display random 7 amino acid peptides-specific antibodies in rheumatoid arthritis sera

WANG Yao1,2,CAI Wen-hui1,YAN Pei-yu1,ZHAI Ai-xia2,WU Jing2,ZHANG Feng-min2(1.Department of Microbiology and Immunology,Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;2.Department of Microbiology,Harbin Medical University,Harbin 150081,China)  
Objective To screen and identify the phage-display random 7 amino acid peptides specific to the antibodies in rheumatoid arthritis(RA) sera and to analyze practical significance of the peptides.Methods The RA specific phage clones were obtained after binding with the mixture of sera from RA patients and normal controls(n=30 per group) as ligand by screening in the phage random 7 peptide library.Then Dot-ELISA was performed to identify the RA specific phage clones reactive to sera of RA patients and normal controls individually;and identified positive phage clones were further screened out by using sera from RA and normal controls(n=12 per group).Finally the identified positive phage clones were sequenced and the homology was also analyzed.Results A total of 17 positive phage clones specific to RA sera were obtained.The reactivity of the mixture of positive phage clones combined with RA sera was significantly higher than that with the normal controls.Sequence analysis showed that there was no consensus sequence between the antigenic epitopes in the identified phage clones.However,the identified phage clones had homology with Bacillus,coccus.Conclusion The antibody content combined with the pathogen epitope exists in the RA sera,indicating that the pathogenesis of RA might be related with the pathogen infection.
【Fund】: 国家863计划项目(2006AA02Z4B1)
【CateGory Index】: R593.22
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