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《Journal of Harbin Medical University》 2012-01
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Muscle-derived stem cells assisted intramuscular fat grafting on mice model

WANG Yan-long,HAN Duan-yang,MA Zhi-qiang,WANG Hai-tao,PIAO Tai-kui,LIU Jian-yu(Department of Orthopedics,The Second Affiliated Hospital of Harbin Medical University,Harbin 150081,China)  
Objective To explore macroscopically and microscopically the auxiliary alienation effect of the MDSCs co-transplanted fat placed intramuscularly.Methods There were A,B and C three groups.Group A(experiment group):MDSCs suspension and autologous fat tissues were co-transplanted into right hind limbs of the recipient mice.Group B(control group):PBS was used to replace the cell suspension and contralateral hind limbs served as the control side.Group C,tibialis anterior muscles from health male adult C57BL/6 mice(15~20 g) were used as blank control.The general observation,MRI scanning and histological staining among groups were compared.Results The volume of the mice hind limbs of experiment group was obviously larger than that of the control groups through the general observation.The MRI scanning showed stronger fat signal of MDSCs-treated group than that of control group.Moreover,histological evaluation exhibited well preserved and intact fall cells in MDSCs-treated group.In contrast,the control group showed extensive fibrosis and fat graft lost.Furthermore,the MDSCs-treated group possessed almost 3-fold of the capillary density to that of the control group.Conclusion Co-transplantation of muscle-derived stem cells and autologous fat tissues improves the long-term survival of intramuscular fat transplants through promoting neovascularization.
【Fund】: 黑龙江省自然科学基金资助项目(D200818)
【CateGory Index】: R622.9
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Li Feng,Jing Wei,Tian Weidong.(Dept.of Traumatic and Plastic Surgery,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China;State Key Laboratory of Oral Diseases,Sichuan University,Chengdu 610041,China);Research progress on microvascular angiogenesis in autologous fat graft[J];International Journal of Stomatology;2012-01
2 Huang Hailing,He jinai,Liu Hongwei,etc;Operation cooperation for fat injection breast augmentation surgery combined by autologous cells injection[J];Journal of Nurses Training;2013-08
3 Brian E.Grottkau,Yunfeng Lin Department of Orthopaedic Surgery,MassGeneral Hospital for Children and the Pediatric Orthopaedic Laboratory for Tissue Engineering and Regenerative Medicine,Harvard Medical School,Boston,Massachusetts,USA;Osteogenesis of Adipose-Derived Stem Cells[J];骨研究(英文版);2013-02
4 LEI Hua, LI Qing-feng( Department of Plastic Surgery, the Ninth People′s Hospital, Shanghai Second Medical University, Shanghai 200011,China);Study on influence of centrifugation on fat viability[J];Chinese Journal of Aesthetic Medicine;2005-01
5 ZHENG Dan-ning, LEI Hua , LI Qing-feng(Department of Plastic Surgery, the Ninth People′s Hospital, Shanghai Second Medical University, Shanghai 200011,China);Study on the effect of several growth factors and DMEM on grafted fat survival[J];Chinese Journal of Aesthetic Medicine;2005-01
6 ;The latest research advancement on fat transplantation[J];Chinese Journal of Aesthetic Medicine;2010-02
7 ;Roles of adipose-derived stem cells in fat transplantation and its clinical application[J];Chinese Journal of Aesthetic Medicine;2010-07
8 ;Current application researches of Adipose-derived stem cells[J];Chinese Journal of Aesthetic Medicine;2010-11
9 TAN Xin-ying1,ZHANG Hai-xia2,LIU Bin3,LIU Yan-pu1,XU Xiao-fang1,LI Long1,LINGHU Da-ke1,XU Hai-yan4(1.Department of Oral and Maxillofacial Surgery,School of Stomatology,The Fourth Military Medical University,Xi'an 710032,Shaanxi,China;2.Department of Stomatology,451 Hospital of PLA,Xi'an 710054,Shaanxi,China;3.Department of Oral Biology and Laboratory Animal Center,School of Stomatology,The Fourth Military Medical University;4.Laboratory Animal Center,School of Stomatology,The Fourth Military Medical University);Experimental study on stromal vascular fraction(SVF) cells to improve the effect of autologous fat tissue transplantation[J];Chinese Journal of Aesthetic Medicine;2011-03
10 ;Status and progress of tissue engineering in plastic Surgery[J];Chinese Journal of Aesthetic Medicine;2011-03
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