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《Journal of Harbin Medical University》 2012-01
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Muscle-derived stem cells assisted intramuscular fat grafting on mice model

WANG Yan-long,HAN Duan-yang,MA Zhi-qiang,WANG Hai-tao,PIAO Tai-kui,LIU Jian-yu(Department of Orthopedics,The Second Affiliated Hospital of Harbin Medical University,Harbin 150081,China)  
Objective To explore macroscopically and microscopically the auxiliary alienation effect of the MDSCs co-transplanted fat placed intramuscularly.Methods There were A,B and C three groups.Group A(experiment group):MDSCs suspension and autologous fat tissues were co-transplanted into right hind limbs of the recipient mice.Group B(control group):PBS was used to replace the cell suspension and contralateral hind limbs served as the control side.Group C,tibialis anterior muscles from health male adult C57BL/6 mice(15~20 g) were used as blank control.The general observation,MRI scanning and histological staining among groups were compared.Results The volume of the mice hind limbs of experiment group was obviously larger than that of the control groups through the general observation.The MRI scanning showed stronger fat signal of MDSCs-treated group than that of control group.Moreover,histological evaluation exhibited well preserved and intact fall cells in MDSCs-treated group.In contrast,the control group showed extensive fibrosis and fat graft lost.Furthermore,the MDSCs-treated group possessed almost 3-fold of the capillary density to that of the control group.Conclusion Co-transplantation of muscle-derived stem cells and autologous fat tissues improves the long-term survival of intramuscular fat transplants through promoting neovascularization.
【Fund】: 黑龙江省自然科学基金资助项目(D200818)
【CateGory Index】: R622.9
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