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《Journal of Harbin Medical University》 2013-01
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In combination with cisplatin to increase durg sensitivity of temozolomide-resistant glioblastoma multiforme cells

LI Jing-bo1,SHI En-hong1,DONG Yu-cui1,JIN Hua3,REN Huan2(1.Department of Anesthesiology,The Second Affiliated Hospital of Harbin Medical University;2.Department of Immunology,Harbin Medical University;3.Department of Cerebral Surgery,The Third Affiliated Hospital of Harbin Medical University,Harbin 150081,China)  
Objective To investigate O6methylguanine-DNA-methyltransferase(MGMT) methylation status mediated temozolomide(TMZ) resistant and evaluate the anti-proliferation effect of TMZ in combination with cell cycle non-specific agent cisplatin(CDDP).Methods Glioblastoma multiforme to be inoculated in DMEM(10% fetal bovine serum).MTT assay was used to determine cell proliferation,methylation-specific polymerase chain reaction(MS-PCR) was applied to detect the methylation status of MGMT gene,Hoechst 33342/PI fluorescent staining was employed to detect the apoptosis,and mechanism of combine medication was evaluated by Chou-Talalay method.Results MGMT was methylated in U87MG which not expressing MGMT protein while incompletely methylated in T98G which expressing MGMT protein.T98G was less sensitive to apoptosis induction following TMZ treatment,while O6-BG improved the sensitivity of T98G cells to TMZ,and Chou-Talalay analysis showed that TMZ combination with CDDP was more sensitive in T98G than U87MG when IC50.Conclusion U87MG which MGMT methylated is more sensitive to TMZ.The combination of TMZ and CDDP can effectively inhibit T98G that MGMT is incompletely methylated.
【Fund】: 黑龙江省科技厅重点项目(ZD200804-01)
【CateGory Index】: R739.4
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