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《Oceanologia Et Limnologia Sinica》 1992-01
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Yan Tao and Zhang Zhirong(South China Sea Institute of Ocfanology, Academta Sinica, Guangzhou 510301)  
The biostimulating effect of low-power laser radiation is a wellknown phenomenon. In order to determine the action of defocusing He-Ne laser irradiation on fishes, a series of experiments were carried out from October 1989 to June 1990. In the study, the parent fishes of guppy, Poecilia reticulata, were taken from Singapore. About two months, they reached sexual maturation and reproduced naturally.The hatched larvae were divided into control and experimental groups randomly. The larvae of the experimental groups were irradiated once every two days and for 25 times altogether. 1. When Trichodina nobillis, Saprolegnia, and Achlya existed in the cultural environment, the survival rate of guppy treated by He-Ne laser at 2.86 J/cm2 was 79.6% after 50 days. But that of the control group was only 35.2% under the same condition.2. The irradiation of He-Ne laser at 5.37 J/cm2 led to the changes of digestive enzymes in 2-month-old guppy. The activity of α-amylase and β-amylase increased by 36.4% and 52.5% respectively, whereas the activity of lipase decreased by 53.5%, and the activity of protease remained at control level. The body length of 3-month-old guppy increased by 8.3% ((?)) or 9.7% ((?)) over the control level, and the body weight increased by 41.7%((?)) or 39.6% ((?)).3. The biostimulating effect of laser light on growth of guppy was not enhanced with the increase of irradiation dosage to 7.52 J/cm2. This may be related to the decrease of the activity of protease.These results demonstrated that He-Ne laser irradiation can enhance disease resistance as well as some metabolic activities and accelerate the growth of guppy at a specific intensity.
【Fund】: 国家计委经费;; 水生生物控制工程技术研究项目 870901号
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