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《OCEANOLOGIA ET LIMNOLOGIA SINICA》 1997-04
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STUDY ON IN-SITU MEASURING SET FOR CHLOROPHYLL-a IN THE SEAWATER

Xia Daying; Wang Zhenxian; Xa Jingfan+; Xia Ronghuan; Xin Haiying(First Institute of Oceanography, SOA, Qingdao 266003)+(Department of physics, Fudan university, Shanghai 200433)  
Ths article describes an in-situ instrument of the chlorophyll-a inseawater which has been successfully developed on the basis of fluorometry. It ismainly used for measuring the chlorophyll-a concentration in phytoplankton in theseasite so that the distribution of sea organisms resources can be assessed. Theinstrument use the pulsed xenon lamp as the excited light resources which has thecharacteristies of low power consumption, high intensity, low repetition rate andnarrow puse width and also use the relevant pulsed detecting technology (i. e. theBoxcar integrating circult) for detecting the fluorescent signal. The signal strength isratioed against the lamp intensity for each flash, by using the detecting methods ofdouble light route and double channel at the same time, so that the accuracy of everymeasuring figure can be ensured, and signal variation due to undulation of the pulsedxenon lamp output intensity can be effectively eliminated. In order to effectivelyseparate the fluorescence produced by the chlorophyll-aof the phytoplankton in thesea which is shone by the excited light and the scatter light from the excied light weuse high block coefficient narmw band filters, the center wavelength of the excitationfilter is 440nm, and the fluorescent emitapce filtors center wavelength is 685nm. Inthis way, the fluorometric signal strength which is received by the receivers can beensured to reflect the fluorescence of chlorophyll-ain the phytoplankton, andfurthermore the interference from the inherent fluorescence in the seawater can beeffectively prevented.The instrument can work from the sea surfuce to a maximum depth of 50m, atany sunlight intensity, for detecting concentration range of chlorophyll-αin thephytoplankon in the ocean from 1 ×10-6- 1×10 9 g / cm3, when the instrument istowed at a speed of 2m/ s, its space resolution is 1m, and this is of practical value to us.
【Fund】: “七五”国家重点科技攻关项目!75-76-04-06号
【CateGory Index】: S969
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