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《Oceanologia Et Limnologia Sinica》 1999-01
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ANALYSIS OF MOLECULAR BIOGEOGRAPHIC MARKER ON RED TIDE TOXIC ALEXANDRIUM TAMARENSE IN THE SOUTH CHINA SEA

CHEN Yue-qin; QIU Xiao-zhong; QU Liang-hu; ZENG Long-mei;QI Yu-zao; ZHENG Lei(School of Life Sciences,School of Chemistry and Chemical Technology, Zhongshan University,Guangzhou,510275)(Institute of Hydrobiology,Jinan University,guanzhou,510632)  
In this study, cultures of eight strains of red the toxic Alexandrium tamarense wereestablished from germinated cysts or vegetative cells isolated from Dapeng Bay, Deya Bay and theHong Kong area in May l992 and December l994, and a method of PCR amplification withindividual cel1s was used to amplify the small subtrit ribosomal RNA genes (Ss-00) of eightstrains for a restriction fragment length Polymorphism (RFLP) assay. PCRRFLP analysis of Ss-rDNAon the red the toxic A lamarense demonstlated that the strains of A lamarense in the South ChinaSea have Ss-rDNA A gene and lack of B gene, which was the same with that of the strains inwestem Japan and Austl-alia (toxic or nontoxic), but different from that of the strains in NorthAlnerican seas. The genetic consistence of toxic A tamarense in China seas suggested that theincrease in the geographic range of A tamarense in ths sea area has Possibly a local origin. Inaddiholl, it was demonstrated that the type of Ss-rDNA could be as a report marker for theidentification and monitoring of the origin and dispersal of toxic species of Alexandrium.
【Fund】: “九五”国家自然科学基金!39790110
【CateGory Index】: Q948
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