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《Oceanologia Et Limnologia Sinica》 1999-01
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WU Xin-zhong; PAN Jin-pei(South China Sea Institute of Oceanology,the Chinese Academy of Sciences,Guanhzhou,510301)  
The morphology, morphogenesis and ultrastructure of Rickettsia-like organism (RLO)inclusion in 5-month old diseased or moribund pearl oysters, Pinctada maxima, collected from theXinying Bay, Hainan Is1and in November 1993 and November 1994 were described. For ahistological examination the mantle, gills, hepatopancreas (including parts of stomoch and inteshne),rectum, adductor muscle, foot muscle and keber's organ were dissected and fixed in Bouln'sfixahve and 4% neutral formalin fixahve. Sechons were swined with rouhne Ehrich's H.E procedureand special methods including Gram-Weigert's stalning and Lehl-Neelsen and Wade-Fite foracid-fast staning. For scanning electron microscopy (SEM) analysis fresh specimens were fixed in2% glutaraldehyde. The selected small pieces (1mm2) of manue hssues were shaved with adoubleedged rasor blade and washed with a sodium cacodylate buffer (three time, 3othen/hme). Driedtissues with the shaved surfaces exposed were sputtercoated with goldpalladium. The characterishcsof RLO inclusion distribuhons in hssues showed that all kjnds of epithelial, connective tissue andendothelial cells are the target cells for RLO infechon. The morphology and size of inclusions fromdifferent tissues are different. The inclusions are Gramnegative but vallable with developing inclusions,Acid-fast-posihve in Which bright red when stained with Wade-Flte's ston and reddish black whenstrined with Ziehl Neelsen's stain.Pregranular and granular stages of developing inclusions were notedunder light microscopic examination. The granular stage may be dishngnished from stage I to stageⅢ (as, to asⅢ). At the pregranular stage (pgi) the inclusions do not contrin granules. Gram stainwas Poor and host cell has sligtihy hypertrophied. From granular stage I to stage Ⅲ, inclusionscontrin developing granules which became more (tens up to 100) and larger (1.0- 4.0u asmeasured in gs Ⅲ inclusions). Gram stain varied from pale red or pale blue to purplish red with b1ueand host cell becoming much more hypertrophied, at last ruptured. Utrastwturaly, twostereo-structure granules, small granules (average 645.85 x 359.O9Um) and large granules (avempe2 090. 93 x 1 556. O8pm), in inclusionpositive host cells were observed under SEM in which somesmall granules were undergoing transverse binary fission (Hg. ltb) and budding division (Hg. I:c),and the large granule was undergoing ruptured with organism escaping (Fig. 1.d). Combined thepresent resu1ts obseried by light and SEM with previous transmission electron microscopy. it issuggested that RLO inclusions consist of two different granules surrounded by a membrane-bound, inwhich the fine granules may be free individual RLO and the large granules may be RLOmicroclonies involved in phagolysosomes.
【Fund】: 中国科学院重点课题!KS85-121
【CateGory Index】: S944.3
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Chinese Journal Full-text Database 9 Hits
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2 ZHU Ning ning,WANG Wen,LI Zheng rong,GU Zhi feng (College of Biological Science,Nanjing Normal University,Nanjing, Jiangsu 210097,China);Ultrastructural observation on hemocytes parasitized by Rickettsia-like organisms of Eriocheir sinensis infected with tremor disease[J];Journal of Chinese Electron Microscopy Society;2002-01
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4 LIANG Yu-Bo, ZHANG Xi-Chang, WANG Li-Jun, YANG Bo, ZHANG Ying, CAI Chu n -Lei (Institute of Marine Environmental Protection, State Oceanic Administration, Dalian, 116023);PREVALENCE OF PERKINSUS SP. IN THE MANILA CLAM RUDITAPES PHILIPPINARUM ALONG NORTHERN COAST OF YELLOW SEA IN CHINA[J];Oceanologia Et Limnologia Sinica;2001-05
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8 WU Xinzhong1,2, PAN Jinpei2, JIANG Jingbo3(1 Marine Biology Research Station at Daya Bay, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;2 South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;3 School of Life Sciences, Zhongshan University, Guangzhou 510275, China);Studies on rickettsialike organism (RLO) diseaseof the tropical marine pearl oyster Ⅶ Epidemiological investigation of RLO disease in larvae populations of maricultured Pinctada maxima[J];Acta Oceanologica Sinica;2002-06
9 WU Xin-zhong, PANJin-pei, JIANG Jing-bo (Zhejing University, Hangzhau 310027, China ;South China Sea Institute of Oceanoiogy , The Chinese Academy of Sciences, Guangzhou 510301, China ;School of Life Sciences, Zhongshan University, Guangzhou 510275, China);Studies on rickettsia-like organism (RLO) disease of tropical marine pearl oyster——Ⅷ. Epidemiological investigation of RLO disease in juvenile populations of maricultured Pinctada maxima[J];Acta Oceanologica Sinica;2003-02
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9 Liu Dejing 1 Wang Jiapan 2 Xiao Hualin 1, Xiao Siqi 1 Huang Tianhua 1 Chi Baoxing 2 (1. Fujian Changle Zhanggang Clam Breeding Farm, Changle, Fujian 350209; 2. Fujian Changle City Fisheries Bureau, Changle Fujian 350200);Studies on the Growth of Coelomactra antiquata (Spengler)[J];Journal of Zhanjiang Ocean University;2003-01
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1 XU Gang-chun1,GU Ruo-bo1,WEN Hai-bo1,HUA Dan1,NIE Chuan2(1.Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agricultural, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081;2.College of Fisheries,Nanjing Agricultural University,Wuxi 214081);Effects of environmental stress on lysozyme and superoxide dismutase of Corbicula fluminea-(Müller)[J];Journal of Anhui Agricultural University;2007-01
2 ZHANG Wei-Zhu 1,4 , WU Xin-Zhong 2 , WANG Ming 3 1. Department of Chemistry and Biology, Chuzhou University, Chuzhou 23900, Anhui, China 2. College of Animal Sciences, Zhejiang University, Hangzhou 310029, China 3. College of Veterinary Medicine, China Agricultural University, Beijing 100094, China 4. South China Sea Institute of Oceanology, the Chinese Academy of Sciences, Guangzhou 510301, China;Surface and ultrastructural characterization of the haemocytes in the scallop Argopecten irradians[J];Acta Zoologica Sinica;2005-03
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