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《Oceanologia et Limnologia Sinica》 2012-01
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EXPRESSION OF METALLOTHIONEIN AND THIOREDOXIN GENE IN CYCLINA SINENSIS EXPOSED TO CADMIUM

Lü Da, LUO Kai-Ya, PAN Bao-Ping, GAO Hong (College of Life Sciences, Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation, Tianjin Normal University, Tianjin, 300387)  
The sequences of metallothionein and thioredoxin of Cyclina sinensis were cloned with the large scale EST sequencing method to built the clams C. sinensis SMART-cDNA library. Real-Time PCR (RT-PCR) technique was used to detect the expression of metallothionein and thioredoxin gene under Cadmium (Cd). The results showed that the full length cDNA of metallothionein and thioredoxin was 776bp and 804bp respectively. The open reading frame (ORF) of metallothionein encoded 74 amino acids (aa) with 7 typical sequence: CXC. The ORF of thioredoxin encoded 165 amino acids with a typical active position (Trp-Cys-Gly-Pro-Cys.) Real-Time PCR showed that the expression of metallothionein gene significant increased under Cd2+ stress from 6h to 12h, which was significant different from the control group (P0.01), then it tend to normal level after 24h. The expression level of thioredoxin gene increased under Cd2+ intimidated at 6h. And the expression of 12h, 24h was significant different from the control group (P0.05). Our investigation showed that Cd2+ could induce the expression of metallothionein and thioredoxin gene, and the transcription reveals the molecular regulation mechanism in the immune defense of Cd2+ stress in clam of Cyclina sinensis.
【Fund】: 天津市科委应用基础与前沿技术重点项目资助 09JCZDJC19300号;天津市科委应用基础研究面上项目资助 10JCYBJC09100号
【CateGory Index】: Q789
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