ANALYSIS OF EXPRESSION AND RECOMBINANT PROTEINS ACTIVITIES OF CATHEPSIN L GENE FROM CYCLINA SINENSIS
LIU Shi-Meng;ZHAO Ting;ZHANG Hao;PAN Bao-Ping;College of Life Sciences,Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University;
Measure the expression of Cathepsin L gene(CsCPL) between organizations in Cyclina sinensis stimulated by Vibrio anguillarum and time-dependent expression in hemolymph with RT-PCR method. The results showed that, CsCPL gene expresses abundantly in hemolymph, liver, mantle, gills and adductor muscle and other tissues in C. sinensis, with the highest expression level in hemolymph. The expression of CsCPL in hemolymph appeared a large number of increasement in 3—96 hours after V. anguillarum stimulation, and at 6 hours reach to maximum which was very significant different(P0.01) with the control group, indicating that CsCPL gene plays an important role in the immune response in C. sinensis. Experiments also carried on the CsCPL genetic prokaryotic expression, and got recombinant protein about 35 kDa molecular weight, which has an obvious bacteriostatic action after purification and renaturation.