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SCREENING OF MICROSATELLITES GENDER-INDICATIVE MARKERS OF TAKIFUGU RUBRIPES

YUE Liang;MA Ai-Jun;ZHAO Yan-Fei;WANG Xin-An;HE Wei-Guo;MENG Xue-Song;ZHAI Jie-Ming;LIU Sheng-Cong;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences; Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology;Fisheries and Life Science,Shanghai Ocean University;Dalian Tianzheng Industrial Co. Ltd.;Laizhou Mingbo Aquatic Products Co. Ltd.;  
To determine the gender differences of male and female populations of Takifugu rubripes, we screened the microsatellites based on bulked segregation analysis(BSA). Two gene pools were constructed with 60 individuals each containing 30 males and females, and scanned by 66 pairs of microsatellite primers, from which eight pairs of microsatellite primers of different bands were amplified and verified for the first round in the 60 individuals. The results show that the bands amplified by primers f1050 and f383 in male and female individuals differed significantly. And in the second round verification, bands amplified by primers f1050 in male and female individuals did not differ significantly, while bands amplified by primers f383 in male and female individuals differed significantly. In other words, primer f383 occurred in male individuals in two rounds of verification in coefficient 0.710 and 0.673, respectively, it is paternity-indicative for T. rubripes. The discovery shall provide a theoretical basis for gender-related gene cloning and sex determination.
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