THE PCR-DGGE ANALYSIS OF FUNGAL COMMUNITY IN THE GILLS OF TWO SHARK SPECIES
ZHANG Yi;MU Jun;FENG Yan;YAN Song;School of Environmental and Chemical Engineering,Dalian Jiaotong University;School of Life Science and Biotechnology,Daian University of Technology;Marine Science and Technology College,Zhejiang Ocean University;
Gill tissue DNA samples were extracted from two shark specimens from East China Sea, Isurus oxyrinchus and Carcharodon carcharias, and used as templates for PCR amplification for the sequences of internal transcribed spacer 1 in ribosomal DNA gene of the fungal communities in the gill tissue. The PCR products were subsequently separated by DGGE(denatured gradient gel electrophoresis). Then, seven main electrophoresis stripes in the spectrum were amplified by secondary PCR, cloned, and sequenced. NCBI-BLAST comparison and molecular phylogenetic analysis indicated that the dominant fungal species in the gills of I. oxyrinchus come from four taxonomical units including one from genus Penicillium and three from Aspergillus. For C. carcharias, one dominant taxonomical unit comes from Penicillium, two from Sterigmatomyces, and one from Acremonium. Penicillium is the common dominant fungal group in gills of the two shark species. In addition, the DGGE fingerprint also shows the presence of diverse minor fungal groups. Database searching suggested that most of these fungal taxonomical units have good potential in the production of natural bioactive substances. The PCR-DGGE analysis revealed that rich fungal community inhabits the two shark specimens' gills. More investigations are demanded for understanding the metabolites and interaction with their hosts.