THE TISSUE DISTRIBUTION AND CELLULAR LOCALIZATION OF ASTAKINE IN SHRIMP LITOPENAEUS VANNAMEI
XU Meng-Lin;LIANG Yan;LU Jin-Feng;WANG Xiao-Wen;LI Chen;XING Jing;HUANG Jie;The Key Laboratory of Maricultur inistry of Education,College of Fisherie,Ocean University of China;Laboratory of Sustainable Development of Marine Fisherie inistry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Science;
Astakine, a cytokine in crustaceans, involves in hematopoiesis for stimulating cell proliferation and differentiation, and plays a key role in crustacean immune responses. In our previous studies, the gene of astakine in shrimp Litopenaeus vannamei(LvAST) was cloned and expressed in vitro. To get more information about the function of LvAST, its tissue distribution was quantified by real time RCR; and additionally, its cellular localization was observed by immunofluorescence method. Results reveald that the Lvast gene distributes widely in different tissues, with relative higher expression in hepatopancreas, muscle, and gills. The cellular location recognized by Dylight 649 labeled polyclonal antibody of LvAST showed that LvAST is expressed not only in hemocytes but also on some hemocytes. In histology immunofluorescence method, we observed that the LvAST protein distributed in hepatopancreas, gills, muscle, and lymphoid organ, which is in accordance with the result of its gene expression in shrimp tissues.