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《Oceanologia et Limnologia Sinica》 2015-01
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MOLECULAR CLONING AND EXPRESSION OF WNT4 GENE IN PACIFIC OYSTER CRASSOSTREA GIGAS

YANG Mei;XU Fei;LIU Jun;LI Li;ZHANG Guo-Fan;National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences;University of Chinese Academy of Sciences;  
As an important member of the Wnt gene family, Wnt4 plays a pivotal role in development processes of many animals. Using the RACE technique, we obtained a complete c DNA sequence of Wnt4 gene in Pacific oyster(Crassostrea gigas). The full length of this gene was 1999 bpencoding 355 amino acids. Homologous analysis on the amino acids showed that C. gigas Wnt4 was over 40% similar to the most species of Wnt4, including Homo sapiens, Platynereis dumerilii, and Chlamys farreri. QRT-PCR results showed that C. gigas Wnt4 gene was expressed in multiple tissues(mantle, gills, labial palp, digestive gland, male gonad, and female gonad), implying that C. gigas Wnt4 plays various biological roles. Among different developmental stages in C. gigas, the early embryogenesis showed high Wnt4 m RNA expression level(highest in the morula stage, followed by gastrula stage), and lowest in the juvenile stage. Therefore, C. gigas Wnt4 may be involved in the formation of certain organs in early developmental stages.
【Fund】: 国家重点基础研究发展计划(973项目) 2010CB126402号;; 国家自然科学基金重点项目 40730845号;; 国家高技术研究发展计划(863项目) 2012AA10A405号;; 贝类产业技术体系项目 CARS-48号;; 泰山攀登计划项目
【CateGory Index】: S917.4
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【Citations】
Chinese Journal Full-text Database 6 Hits
1 TAO Li-Hong,YAO Li-Xiao,FU Zhi-Qiang,FENG Xin-Gang,LIU Jin-Ming,SHI Yao-Jun,YUAN Chun-Xiu+*, CAI You-Min and LIN Jiao-Jiao+*Shanghai Veterinary Research Institute of CAAS, National Laboratory of Animal Schistosomiasis Contronl, Key Laboratory of Animal Parasitology Ministry of Agriculture, Shanghai 200232, China;Cloning, Expression and Characterization of a Gene Encoding Signal Transduction Protein Wnt4 of Schistosoma japonicum[J];Chinese Journal of Biotechnology;2007-03
2 LI Jian-Zhong 1,2,LIU Qiong 1,WANG De-Shou 2,ZHOU Lin-Yan 2,SAKAI Fumie 2 and NAGAHAMA Yoshitaka 2(1.Key Lab of Protein Chemistry and Developmental Biology of Ministry of Education,College of Life Science,Hunan Normal University,Changsha 410081,China;2.Lab of Reproductive Biology,National Institute for Basic Biology,Okazaki 444-8585,Japan);MOLECULAR CLONING AND IDENTIFICATION OF TWO WNT4 GENES FROM THE MEDAKA (ORYZIAS LATIPES)[J];Acta Hydrobiologica Sinica;2012-05
3 Ding-Zi Yin,Hai-Yun Song~* (Key Laboratory of Systems Biology,Chinese Academy of Sciences,Shanghai 200031,China);Regulation of Wnt Signaling:Mechanisms and Biological Significance[J];Chinese Journal of Cell Biology;2011-02
4 QIAN Guang-Hui,WANG Yi-Quan School of Life Sciences,Xiamen University,Xiamen 361005,China;Wnt signaling pathway and the Evo-Devo of deuterostome axis[J];Hereditas;2011-07
5 HAN Lin~1,FENG Xin-gang~2(1.Life and Environment College of Shanghai Normal University,Shanghai 200234,China;2.Shanghai Institute of Veterinary Science,CAAS,Shanghai 200232,China);WNT SIGNALING PATHWAY AND ITS ROLE DURING ANIMAL'S GROWTH AND DEVELOPMENT[J];Chinese Journal of Veterinary Parasitology;2008-03
6 LI Hailong,LIU Jianguo,LIU Xiaoling,ZHANG Zhifeng Key Laboratory of Marine Genetics and Breeding of Ministry of Education,Ocean University of China,Qingdao 266003,China;Molecular cloning and expression analysis of wnt4 cDNA from the Zhikong scallop Chlamys farreri[J];Journal of Fishery Sciences of China;2013-02
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Chinese Journal Full-text Database 10 Hits
1 ZHENG Ji-meng;LIU Ying;Institute of Oceanology,Chinese Academy of Sciences;University of Chinese Academy of Sciences;;Study on the Effects of Bacillus pumilus and Rhodotorula mucilaginosa on the Early Cultivation of Scapharca broughtonii Seedlings[J];Journal of Anhui Agricultural Sciences;2014-23
2 ZHANG Yuan;ZHU Ying;Department of Infections,the First Affiliated Hospital of Dalian Medical University;;Efficacy studies of Yiguanjian treatment in mice fibrosis induced by dimethylnitrosamine[J];Journal of Dalian Medical University;2014-06
3 YE Quan-tu;WU Qi-sheng;ZENG Zhi-nan;SU Xin-hong;LIU Yong;Fisheries Research Institute of Fujian;;Effect of different bottom sowing density on growth and survival of Babylonia lutosa juveniles[J];Journal of Fujian Fisheries;2015-01
4 QIN Yu,GUO Xin-biao,DENG Fu-rong.Department of Occupational and Environmental Health Sciences,School of Public Health,Peking University,Beijing 100191,China;Research advances in regulation of gap junctional intercellular communication[J];Journal of Environment and Health;2012-09
5 ;Wnt signaling pathway and wound healing[J];Chinese Journal of Aesthetic Medicine;2012-07
6 ZHOU Chun-Ya;ZHU Ling;PAN Ying;XIE Ming-Song;YANG Ao-Ao;CHEN Si-Qing;ZHUANG Zhi-Meng;College of Fisheries and Life Science,Shanghai Ocean University;Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Shandong Provincial Key Laboratory of Fishery Resources and Eco-environment,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;;Wnt5 GENE FROM RHOPILEMA ESCULENTUM:cDNA CLONING,GENOMIC ORGANIZATION AND mRNA EXPRESSION[J];Oceanologia et Limnologia Sinica;2013-04
7 LI Zhi-min;LIU Zhi-gang;LIANG Chun-qiao;PENG Hai-long;SONG Hui-ge;QIN Yan-ping;LEI Du-yun;Fisheries College,Guangdong Ocean University;;Investigation of culture environment in inshore and offshore pearl-cultivating sea areas of Liusha bay[J];Marine Sciences;2014-02
8 ZHONG Fang-jie;YAN Xue-yu;GAO Yang;JIANG Qing-yan;JIA Zheng;LI Qiong-zhen;LI Wen-hong;CHEN Qin;College of Animal Science and Technology of Guangxi University;Guangxi Institute of Fisheries;;Species identification species group structure analysis of the common oysters from the Maowei sea in Guangxi[J];Marine Sciences;2014-03
9 YANG Zhenxiong;MAO Yangli;SONG Na;GAO Tianxiang;CAI Houcai;ZHANG Zhaohui;Institute of Evolution & Marine Biodiversity,Ocean University of China;South China Sea Environmental Monitoring;Nanji Islands National Marine Nature Reserve Research Institute;First Institute of Oceanography,SOA;;Sequence Analysis of Mitochondrial Coi Gene of Mytilus Coruscus Along Chinese Coast Waters[J];Transactions of Oceanology and Limnology;2014-02
10 BO Qi-Kang;ZHENG Xiao-Dong;WANG Pei-Liang;BI Ke-Zhi;Fisheries College,Ocean University of China;Mashan Group Co.Ltd.;Rongcheng Green Food Office;;BASIC GROWTH RELATIONS IN EXPERIMENTAL REARING OF NEWLY HATCHLINGS OF OCTOPUS MINOR(SASAKI,1920)[J];Oceanologia et Limnologia Sinica;2014-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 TAO Li-Hong,YAO Li-Xiao,FU Zhi-Qiang,FENG Xin-Gang,LIU Jin-Ming,SHI Yao-Jun,YUAN Chun-Xiu+*, CAI You-Min and LIN Jiao-Jiao+*Shanghai Veterinary Research Institute of CAAS, National Laboratory of Animal Schistosomiasis Contronl, Key Laboratory of Animal Parasitology Ministry of Agriculture, Shanghai 200232, China;Cloning, Expression and Characterization of a Gene Encoding Signal Transduction Protein Wnt4 of Schistosoma japonicum[J];Chinese Journal of Biotechnology;2007-03
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