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《Oceanologia et Limnologia Sinica》 2016-01
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DEVELOPMENTAL STAGES OF A MARINE MODEL FISH ——MEDAKA ORYZIAS MELASTIGMA

CHEN Yi;WANG Xiao-Jie;RAN Hao-Yu;LIN Yu;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources;International Center of Marine Study;Institute for Marine Biosystem and Neurosciences, Shanghai Ocean University;  
Marine medaka(Oryzias melastigma) is a promising potential model organism for ecotoxicology in marine environment. They are sensitive to pollutants in the early development. Therefore, we shall understand the embryo toxicity in the fish to establish an applicable standard for toxicity assessment. However, the embryonic development of it has not been reported. In this study, we investigated the reproductive habits and the stages of development of the fish in normal conditions. Fertilized eggs of O. Melastigma were incubated at(26±0.5)°C, and they were hatched out in 220 h 30min, during which the process of embryonic development was carefully observed with a stereomicroscope. The embryonic development could be divided into 8 stages and 33 substages in diagnostic features of developing embryos. The major stages include those of fertilized egg activation, blastodisc formation, egg cleavage, blastula, grastula, neurula, organogenesis, and hatching out.
【Fund】: 国家自然科学基金项目 41306097号;; 中国科学院海洋生态与环境科学重点实验室开放课题 KLMEES2013号;; 上海高校水产学一流学科建设资助项目;; 国际海洋研究中心项目
【CateGory Index】: S917.4
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