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《Oceanologia et Limnologia Sinica》 2016-01
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DNA BARCODING FOR WORLDWIDE HAEMULINAE IN CLASSIFICATION AND PHYLOGENY

LIANG Ri-Shen;WU Li-Yu;SU Guo-Mao;CHENG Shuo;ZHOU Meng;WU Zao-He;College of Life Science, Zhongkai University of Agriculture and Engineering;  
To investigate the feasibility of using COⅠ sequences as DNA barcodes to identify Haemulinae species, we determined the 651 bp COⅠ sequences of 91 Haemulinae individuals sampled from 7 genera 29 species, calculated the genetic distances inter- and intra-species using MEGA 5.0, and constructed the molecular phylogenetic trees using maximum parsimony and maximum likelihood methods. The result show that the inter-species genetic distance was 0.021—0.240 in average of 0.184, while that of intra-species was 0.000—0.009 in average of 0.004. The average inter-species value(0.184) is 46 times larger than that of intra-species one(0.004). Moreover, each inter-species genetic distance value is larger than the minimum species identification value 0.200(2%) suggested by Hebert. In the molecular phylogenetic trees, individuals of each species formed an independent monophyletic group, indicating that COⅠ could be used as an effective barcode gene for accurate identification among Haemulinae species. Meanwhile, as the phylogenetic trees show, the Pomadasys fish are non-monophyletic and form 5 independent groups. Such phylogenetic relationships are due to their geographic distribution, which is consistent with the viewpoint of modern molecular phylogeography studies.
【Fund】: 广东省教育厅青年创新人才类项目 2014KQNCX164号;; 广东省大学生创新创业训练计划项目 201511347028号;; 仲恺农业工程学院大学生创新基金项目 2015A08号 2015A10号
【CateGory Index】: S917.4
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