APPLICATION OF HIGH-THROUGHPUT SEQUENCING FOR ANALYZING BACTERIAL COMMUNITIES IN EARTHEN PONDS OF SEA CUCUMBER AQUACULTURE IN NORTHERN CHINA
DOU Yan;ZHAO Xiao-Wei;DING Jun;HE Peng;Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University;
Sea cucumber Apostichopus japonicus has become an economically valuable holothurian species because of their anti-tumor properties and good nutritional values. We applied high-throughput sequencing to explore bacterial community in earthen aquaculture ponds of the sea cucumber in Dalian, North China. For nine samples collected, the number of effective sequences ranged 26503––37825, and they could be classified into 1502—5741 operational taxonomic units. Results show great bacterial diversities in the aquaculture. The estimated richness and diversity indices were higher in sediment samples than those in water samples. In total, 26 phyla were identified, among which Proteobacteria, Bacteroidetes, Chloroflexi, Planctomycetes, Cyanobacteria, Acidobacteria, Verrucomicrobia, Firmicutes, and Actinobacteria were commonly reported, and other 17 were firstly detected and rarely reported. Despite the differences in microbe composition, Proteobacteria and Bacteroidetes(50.54%—99.91%) were the dominant phyla in all samples.