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《Oceanologia et Limnologia Sinica》 2016-01
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MOLECULAR IDENTIFICATION AND DETECTION OF CYANEA NOZAKII BY PARTIAL SEQUENCING OF 18S RDNA AND ITS-5.8S

LI Yu-Long;DONG Jing;WANG Bin;SUN Ming;WANG Ai-Yong;WANG Wen-Bo;Liaoning Ocean and Fisheries Science research Institute, Key Laboratory of Marine Biological Resources and Ecology;  
We determined partial sequences of nuclear 18 S and ITS-5.8S r DNA from polyp, ephyra, and adult of jellyfish Cyanea nozakii, and compared them with available homologous sequences of other Cyanea jellyfish species in the Gen Bank in terms of sequence variation and genetic distance. Result shows that the nucleotide divergence in ITS-5.8S sequence was 0%–0.8% between C. nozakii and uncultured eukaryote species from the Gen Bank, suggesting that the uncultured eukaryote species was C. nozakii. In 18 S r DNA, a 1709-bp homologous sequence was recognized at 33 variable sites in five Cyanea jellyfish species, while in ITS, a 368-bp long homologous sequence was shown including 203 variable sites, 178 parsimony informative sites, and 21 singleton sites. The average genetic distance inter- / intra-species was 0.008 / 0, and 0.284 / 0.019 in 18 S r DNA and ITS1, respectively, showing that ITS1-based inter-species genetic distance was 15 times greater of inter-species one; and therefore the former is more suitable for species identification. Moreover, phylogenetic trees were constructed in the Neighbor-Joining method, from which different individuals of the same species grouped together. Therefore, the ITS1 gene fragments were highly diversified among different Cyanea species and could be applied to identify large-sized jellyfish and detect young jellyfish, and are helpful in molecular systematic studies at species or population level.
【Fund】: 国家自然科学基金项目资助 31400406号;; 国家重点基础研究发展计划(973)项目 2011CB403601号;; 海洋公益性行业科研专项资助 201405010号
【CateGory Index】: S917.4
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