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《Oceanologia et Limnologia Sinica》 2016-01
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BIOSYNTHEZIED SILVER NANOPATICLES USING HAWTHORN FRUIT EXTRACT AND THEIR ANTIBACTERIAL ACTIVITY AGAINST FOUR COMMON AQUATIC PATHOGENS

JIANG Yu;LI Fu-Yan;LIU Chong-Chong;XU Hui;SUN Hu-Shan;WANG Lei;College of Life Sciences, Ludong University;College of Chemistry and Material Sciences,Ludong University;  
"Green" synthetization of nano-antibacterial materials has become a promising strategy in nanotechnology in the past few years because of its simple, low-cost, and eco-friendly advantages. To study the antibacterial effect of biosynthesized silver nanoparticles(Ag NPs) against aquatic pathogenic bacteria, we synthesized Ag NPs using water and ethanol extracts of hawthorn fruit as reducing and stabilizing agent, and named them as W-Ag NPs and E-Ag NPs, respectively. In addition, we studied the formation of W-Ag NPs and E-Ag NPs in the surface plasmon resonance band illustrated by UV-vis spectrophotometer, observed their morphology in transmission electron microscopy, and investigated the crystalline structure of them in X-ray diffraction. Moreover, we conducted a preliminary antimicrobial study on W-Ag NPs and E-Ag NPs against two common pathogens: Escherichia coli and Staphylococcus aureus. The results showed that W-Ag NPs was smaller and more homogeneous in size, moreover, it had higher antibacterial activity. Further antibacterial study showed that W-Ag NPs possessed remarkable antibacterial activity against all aquatic pathogens tested, including Vibrio anguillarum, Vibrio anguillarum, Vibrio parahaemolyticus, and Aeromonas punctata. As shown in bacteriostasis kinetic curves, W-Ag NP concentrations at 10 and 20 μg/m L could significantly inhibit the growth of V. anguillarum. The minimum inhibitory concentration(MIC) and the minimum bactericidal concentration(MBC) of W-Ag NPs against V. anguillarum were 6.5 and 12.9 μg/m L, respectively. Therefore, biosynthesized Ag NPs using water extract of hawthorn fruit can be used as an effective and broad-spectrum antibacterial agent in promising potential for aquaculture.
【Fund】: 国家自然科学基金面上项目 31570649号;; 山东省自然科学基金面上项目 ZR2013CM018号;; 山东省高校科研计划项目 J15LE03号
【CateGory Index】: S941.4
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