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《Marine Sciences》 2013-01
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An efficient methodology of sperm cryopreservation in Centropristis striata

LIU Hong-jun1,GUAN Shu-guang1,LIU Qing-hua2,LI Jun2,YU Dao-de1(1.Mariculture Institute of Shandong Province,Qingdao 266002,China;2.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China)  
In the present study,Centropristis striata sperm was efficiently cryopreserved using a programmable freezer.The motility of both fresh and post-thaw sperm was investigated in order to optimize the sperm cryopreservation protocols for C.striata.Four cryoprotectants(15% dimethyl sulfoxide,15% ethylene glycol,15% propylene glycol and 10% methanol),five cooling rates(10,15,20,25 and 30℃/min) as well as five thawing temperatures(30,35,40,45 and 50 ℃) were designed and tested in the sperm cryopreservation,and their effects on post-thaw sperm motility were studied.Optimal post-thaw motility(60%) were achieved when using Hanks’s supplemented with 15% DMSO,15% PG and 15% EG with 30℃/min cooling rate,and 35℃ water bath.An efficient cryopreservation method for C.striata sperm was established by analyzing the effect of cryoprotectants,cooling rates and thaw temperatures.This method is advantageous not only to establish laboratory experiment but also to preserve genetic resources for routine aquaculture hatchery operation.
【Fund】: 农业部948项目(2011-Z39);; 中国科学院海洋研究所知识创新工程领域前沿项目(Y02507101Q);; 国家自然科学基金资助项目(41076100 31072212)
【CateGory Index】: S961.2
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 YE Ting1, ZHU Jun-quan1, , YANG Wan-xi2, WEI Pin1, WU Xiong-fei3 * (1. Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University, Ningbo 315211, China; 2. College of Life Science, Zhejiang University, Hangzhou 310012, China; 3. Ningbo Academy of Oceanology and Fisheries, Ningbo 315012, China);Sperm Cryopreservation in Sparus macrocephalus and DNA Damage Detection with SCGE[J];Zoological Research;2009-02
2 LI Chun LI Jun XUE Qin zhao(Institute of Oceanology, Chinese Academy of Sciences Qingdao, 266071);CRYOPRESERVATION OF SPERMATOZA OF RED SEA BREAM[J];Marine Sciences;2001-12
3 XIAO Zhi-zhong1,CHEN Xiong-fang2,DING Fu-hong1,3,LIU Qing-hua1,3,XU Shi-hong1,LI Jun 1(1.Center of Marine Biotechnology Research & Department,Institute of Oceanology,the Chinese Academy of Sciences,Qingdao 266071,China;2.Chongqing College of Posts and Telecommunications,Chongqing 400065,China;3.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China);An efficient methodology of sperm cryopreservation of large yellow croaker(Pseudosciaena croce)[J];Marine Sciences;2007-04
4 LEI Ji-lin1 LU Ji-wu2(1Yellow Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071)(2Qingdao Seacul Turbot Research Institute Co. Ltd, 266002);The breed predominance and culture prospects of Centropristis striata Linnaeus[J];Marine Fisheries Research;2007-05
5 LIN Danjun,YOU Yonglong(Department of Biology,Fujian Normal University,Fuzhou 350007,China);PHYSIOLOGICAL CHARACTERISTICS AND CRYOPRESERVATION OF PSEUDOSIAENA CROCEA(RICHARDSON) SPERMS[J];Tropic Oceanology;2002-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XU Hai-long et al (Key Laboratory of Aqua-ecology and Aquaculture,Tianjin Agricultural College,Tianjin 300384);Study on Cryopreservation of Scortum barcoo Spermatozoa[J];Journal of Anhui Agricultural Sciences;2010-06
2 YANG Pei-min1,2,YANG Ai-guo1,LIU Zhi-hong1,ZHOU Li-qing1(1.Key Laboratory for Sustainable Utilization of Marine Fisheries Resource,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.Freshwater Fisheries Science Institute of Liaoning Province,Liaoyang 111000,China);Physiological Characteristics and Short-term Storage of Yesso Scallop Patinopecten yessoensis Sperm[J];Fisheries Science;2008-09
3 DANG Ran1,ZHU Jun-quan1,QIU Xin-zhi2(1.Key Laboratory of Applied Marine Biotechnology of the Ministry of Education,Ningbo University,Ningbo 315211,China;2.Ningbo International Invest and Consult Corporation Ltd.,Ningbo 315010,China);Analysis of flesh content and nutrient components in the muscle of Centropristis striata[J];Journal of Marine Sciences;2010-02
4 YE Ting1, ZHU Jun-quan1, , YANG Wan-xi2, WEI Pin1, WU Xiong-fei3 * (1. Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University, Ningbo 315211, China; 2. College of Life Science, Zhejiang University, Hangzhou 310012, China; 3. Ningbo Academy of Oceanology and Fisheries, Ningbo 315012, China);Sperm Cryopreservation in Sparus macrocephalus and DNA Damage Detection with SCGE[J];Zoological Research;2009-02
5 WANG Xiao-Ai1,2,YANG Jun-Xing1,*,CHEN Xiao-Yong1,*,PAN Xiao-Fu1,LI Zai-Yun1(1.State Key Laboratory of Genetic resources and Evolution,Kunming Institute of Zoology,the Chinese Academy of Sciences,Kunming 650223,China;2.Graduate School of the Chinese Academy of Sciences,Beijing 100049,China);Cryopreservation of sperm from Neolissochilus benasi[J];Zoological Research;2012-03
6 LIN Xing(Putian Institute of Bio-engineering,Putian,Fujian 351100,China);The optimum nutrient requirements of protein,lipid and energy-protein ratio in formulated feed in juvenile Centropristis striata[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2011-04
7 LIN Guo-qing(Fujian Fishery Technical Extension Center,Fujian 350003,China);Studies on embryonic development and scale manual breeding techniques of Centropristis Striata[J];Journal of Fujian Fisheries;2012-01
8 LIN Dan-jun,YOU Yong-long,CHEN Bing-Ying(College of Life Sciences,Fujian Normal University,Fuzhou 350007,China);Variation of the Motility and Ultrastructure of the Frozen-thawed Sperms in Teleost,Pseudosiaena crocea(Richardson)[J];Journal of Fujian Normal University(Natural Science Edition);2006-03
9 SHU De-bin,GUO Bai-fu,WAN Jian-yi,TIAN Jia-yuan(Chinese Sturgeon Research Institute,China Three Gorges Corporation,Yichang 443100,China);Cryopreservation of Sperm in Paddlefish(Polyodon spathula)[J];Fisheries Science;2013-06
10 LIN Bin, HUANG Zongwen, LUO Jian*, GUO Renxiang, YANG Wei, CHEN Guohua (College of Ocean,Hainan University;Key Laboratory of Tropic Biological Resources of Ministry of Education,Haikou 570228,China);Embryonic Development Observation of Epinephelus Fuscoguttatus[J];Journal of Hainan Normal University(Natural Science);2010-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN SONG-LIN, LIU XIANG-TING, LU DA-CHUN, ZHANG LONG-ZHEN, FU CAO-JUN AND FANG JIAN-PING(Chang Jiang Fisheries Research Institute, Shashi, Hubei 434000);CRYOPRESERVATION OF SPERMATOZOA OF SILVER CARP, COMMON CARP, BLUNT SNOUT BREAM AND GRASS CARP[J];Acta Zoologica Sinica;1992-04
2 WANG Hong-tian; ZHANG Pei-jun(Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071);EFFECTS OF ENVIRONMENTAL FACTORS ON SPERM MOTILITY IN FLOUNDER, PARALICHTHYS OLIVACEUS (T. ET S.)[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1999-03
3 Jiang Shigui 1, Li Jiaer 1, Ou Youjun 1, Zhong Chuangguang 2 and Yu Dahui 1 ( 1South China Sea Fish, Institute, CAFS, Guangzhou, 510300) ( 2Biology Department, Zhongshan University, Guangzhou, 510275);STUDIES ON HYBRIDIZATION BETWEEN FEMALE SILVER BREAM Rhabdosargus sarba AND MALE RED BREAM Pagrosomus major[J];MARINE SCIENCES;1997-05
4 LI Chun LI Jun XUE Qin zhao(Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071);CRYOPRESERVATION OF SPERMATOZA OF BLACK PORGY[J];Marine Sciences;2001-11
5 LI Chun LI Jun XUE Qin zhao(Institute of Oceanology, Chinese Academy of Sciences Qingdao, 266071);CRYOPRESERVATION OF SPERMATOZA OF RED SEA BREAM[J];Marine Sciences;2001-12
6 XIAO Zhi-zhong1,CHEN Xiong-fang2,DING Fu-hong1,3,LIU Qing-hua1,3,XU Shi-hong1,LI Jun 1(1.Center of Marine Biotechnology Research & Department,Institute of Oceanology,the Chinese Academy of Sciences,Qingdao 266071,China;2.Chongqing College of Posts and Telecommunications,Chongqing 400065,China;3.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China);An efficient methodology of sperm cryopreservation of large yellow croaker(Pseudosciaena croce)[J];Marine Sciences;2007-04
7 Hai Ou LU, Li ZHANG, Ning ZHANG, Jie TANG, Xiao Ping DING, Yi TANG (Reproduction Centre, 202 Hospital of PLA, Shenyang, Liaoning 110003, China);Detection of DNA Damage of Human Sperm Using Single Cell Electrophoresis[J];Acto andrologica Sinica;2002-06
8 OU Youjun; Li Jiaer; Jiang Shigui (South China Sea Fisheries Institute, The Chinese Academy of Fishery Sciences, Guangzhou 510300);EFFECTS OF PRESERVATION AND ACTIVATION ON BIOLOGICAL PROPERTY OF PAGROSOMUS MAJOR (TEMMINCK ET SCHLEGEL) SPERMATOZOA[J];TROPIC OCEANOLOGY;1998-03
9 Jiang Shigui Ou Youjun Li Jia’er (South China Sea Fisheries Institute, CAFS, Guangzhou 510300);EFFECTS OF PRESERVATION UNDER DIFFERENT TEMPERATURES ON BIOLOGICAL PROPERTY OF BLACK PORGY (SPARUS MACROCEPHALUS) SPERM[J];TROPIC OCEANOLOGY;1999-04
10 LIN Danjun,YOU Yonglong(Department of Biology,Fujian Normal University,Fuzhou 350007,China);PHYSIOLOGICAL CHARACTERISTICS AND CRYOPRESERVATION OF PSEUDOSIAENA CROCEA(RICHARDSON) SPERMS[J];Tropic Oceanology;2002-04
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