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《Marine Sciences》 2018-06
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Infection kinetics of GFP-Labeled Edwardsiella tarda in Mugilogobius chulae

YU Lu-jun;LI Jian-jun;WEI Yuan-zheng;CAI Lei;MIAO Zong-yu;HUANG Ren;Guangdong Laboratory Animals Monitoring Institute, Guangdong Provincial Key Laboratory of Laboratory Animals;  
Edwardsiella tarda is an important fish pathogen that causes septicemia in many marine and freshwater fish. In order to study the invasion pathway of E. tarda in Mugilogobius chulae and provide basic data on pathogenesis of E. tarda. The infection kinetics of E.tarda were investigated in vivo in M. chulae using a virulent strain, E.tarda EtMc1512, that was transformed with a plasmid encoding green fluorescent protein(PMDpp-EGFP) by electroporation. Fish were divided into three groups: bacteria bath-challenged fish, bacteria intraperitoneally-challenged fish, bacteria intramuscularly-challenged fish. The number of bacteria from blood, gills, liver, intestine and muscle were detected at 0, 2, 4, 6, 12, 24 and 48 h after immersion challenge, which were also detected at 6, 12, 24, 48, 72 and 96 h after intraperitoneal and intramuscular challenge. The results showed that EtMc1512-GFP strain had the similar virulence characteristics as the parent strain and could be easily identified as bright green fluorescing colonies. The virulence-related genes(citC, mukF, esrB, katB, fimA, gadB) have been detected simultaneity in strain EtMc1512 and EtMc1512-GFP. High numbers of bacteria were observed in the intestine(2.51×106 CFU/g), followed by the gills(4.19×104 CFU/g) and blood(1.65×104 CFU/g) of fish after immersion challenge. The number of bacteria was significantly higher in the intestine than other tissues(P0.05). The highest numbers of bacteria were observed in the blood(4.65×106 CFU/g) and liver(4.55×106 CFU/g) of fish after intraperitoneal challenge. Bacteria was first detected in muscle(48 h) and the highest numbers of bacteria were observed in the blood(2.93×104 CFU/g) of fish after intramuscular challenge. These results indicate that the intestine, liver and muscle serve as an important infectious route of E.tarda in M. chulae after immersion, intraperitoneal and intramuscular challenge respectively. It would be high infection risk through the ingestion of contaminated food and water for M. chulae under natural conditions.
【Fund】: 国家科技支撑计划项目(2015BAI09B05);; 广东省科技计划项目(2017B030314171 2017A070702001)~~
【CateGory Index】: S943
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【Citations】
Chinese Journal Full-text Database 6 Hits
1 LU Chunyun;XIE Jun;XI Bingwen;LIANG Liguo;XIA Fei;PAN Liangkun;Wuxi Fisheries College, Nanjing Agricultural University;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture,Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;;Infection kinetics of Aeromonas hydrophila in the blunt-snout bream, Megalobrama amblycephala[J];中国水产科学;2015-05
2 LI Ning-qiu,YU Lu-jun,FU Xiao-zhe,LIU Li-hui,LIN Qiang,CHANG Ou-qin,SHI Cun-bin,HUANG Zhi-bin,WU Shu-qin(Key Laboratory of Fishery Drug Development,Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology,Guangdong Province,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China);Construction of Edwardsiella tarda ghosts from eel and their optimization of preparation conditions[J];水产学报;2012-11
3 SU You-lu,FENG Juan,GUO Zhi-xun,XU Li-wen,WANG Jiang-yong(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences Guangzhou 510300,China);Histopathological analysis of golden pompano Trachinotus ovatus infected with Photobacterium damselae subsp. piscicida[J];海洋科学;2012-02
4 YAN Yun-feng1,2,SU You-lu1,GUO Zhi-xun1,XU Li-wen1,FENG Juan1,HUANG Jian-nan1,YU Da-hui1 (1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;2.Shanghai Ocean University,Shanghai 200090,China);The research on pathogenicity of fish nervous necrosis virus to Rachycentron canadum juvenile[J];海洋科学;2010-04
5 LI Jing,ZHANG Xiao-lu,LIU Yong-jie,LU Cheng-ping(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China);Genetically marking of Aeromonas hydrophila strains with green fluorescent protein gene[J];畜牧与兽医;2009-03
6 Jiang Yun1 Li Shousong2 Wang Shoukun1 Guo Lixin2 Jiang Shuxun2 Chen Wenbing2 Shao Biying2(1The Animal Science College of Fujian Agricultural and Forestry University,Fuzhou 350002 2Fujian Entry-exit Inspection and Quarantine Bureau of the P.R.C.,Fuzhou 350001);Detection of the Virulence Genes of Pathogenic Edwardsiella Tarda by PCR Assay[J];中国食品学报;2008-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 AI Kete;XU Pei;ZHOU Jingwen;WAN Jing;YANG Daiqin;XU Qiaoqing;School of Animal Science,Yangtze University;Hubei Collaborative Innovation Center for Freshwater Aquaculture;;Establishment and Application of Multiplex PCR Assay for Detection of Five Freshwater Bacterial Species[J];水产科学;2015-05
2 ZHU Wen-jin;CHEN Cui-zhen;SU Yong-mei;GE Mu-xiang;ZHANG Yan-ying;JING Xiao-min;FANG Hai;Hebei Normal University of Science and Technology,Key Laboratory of Preventive Veterinary Medicine of Hebei Province;;Detection and Sequencing of Virulence Genes of Pathogenic Edwardsiella tarda in Paralichthys olivaceus[J];动物医学进展;2014-11
3 LI Ningqiu;YU Lujun;FU Xiaozhe;LIU Lihui;LIN Qiang;ZHANG Defeng;SHI Cunbin;WU Shuqin;Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Fishery Drug Development,Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology,Guangdong Province;;Immune efficacy of Edwardsiella tarda ghosts vaccine for European eel (Anguilla anguilla) by 3 kinds of inoculation routes[J];水产学报;2014-11
4 ZHAO Ran;CHEN Qiong;KONG Fan-de;XU Shu-fei;YANG Tao;XU Shu-juan;Xiamen Agricultural Product Quality and Safety Testing Center;Xiamen Entry-Exit Inspection and Quarantine Bureau;;Detection of Four Pathogenic Entero-Bacteria in Food with Four Real-Time Fluorescent Quantitative PCR Technique[J];经济动物学报;2014-03
5 LIU Xiao-Dan;HU Xian-Qin;HUANG Jian-Nan;WENG Shao-Ping;WANG Wen-Wen;CHEN Wen-Jie;QIN Zhen-Dong;DONG Xing-Xing;ZHOU Yang;LIU Xiao-Ling;JI Wei;ZHANG Xue-Zhen;GUO Zhi-Xun;HE Jian-Guo;LIN Li;Department of Aquatic Animal Medicine,College of Fisheries,Huazhong Agricultural University;School of Life Sciences,Sun Yat-Sen University;Key Lab of Freshwater Animal Breeding,Ministry of Agriculture;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;Hubei collaborative Innovation Center for Freshwater Aquaculture;;DISTRIBUTION OF NERVOUS NECROSIS VIRUS IN THE JUVENILE RED-SPOTTED GROUPERS(EPINEPHELUS AKAARA) BY IN SITU HYBRIDIZATION AND IMMUNOFLUEORESCENT ASSAYS[J];水生生物学报;2014-05
6 LIU Tao;WANG Kai-yu;GENG Yi;CHEN De-fang;CHEN Cheng;YU Ze-hui;PU Yun-dan;XU Jing-jun;ZHOU Yan;WANG Jun;Fisheries Research Department,Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province,Sichuan Agricultural University;;Identification of Edwarsdsiella tarda from infected Schizothorax prenanti[J];中国预防兽医学报;2014-08
7 ZHENG Feng-rong;CHEN Hao-wen;First Institute of Oceanography,SOA;;Analysis Achievements of Marine Microbiological in China During 2012 and 2013[J];广西科学院学报;2014-02
8 WU Shuqin;TAO Jiafa;GONG Hua;CHANG Ouqin;REN Yan;LIU Lihui;CHEN Zonghui;LAI Yingtiao;Key Laboratory of Fishery Drug Development,Ministry of Agriculture; Key Laboratory of Aquatic Animal Immune Technology,Pearl River Fishery Research Institute,Chinese Academy of Fishery Sciences;;Situation and tendency of the fishery vaccine development[J];中国渔业质量与标准;2014-01
9 JIANG Na;XING Wei;MA Zhi-hong;LI Tie-liang;LUO Lin;Beijing Fisheries Research Institute,Beijing Academy of Agriculture and Forestry Sciences;;Research Progress in Aquaculture Ghost Vaccines[J];中国生物工程杂志;2013-11
10 GENG Wei-Guang;SHI Cheng-Yin;LI Jin;HUANG Jie;WANG Xiu-Hua;XIE Guo-Si;Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture; Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;College of Fisheries and Life Science,Shanghai Ocean University;;Establishment and Application of Amplicon Rescue Multiplex PCR(Arm-PCR) for the Simultaneous Detection of Five Major Bacterial Pathogens from Marine Aquaculture Animals[J];农业生物技术学报;2013-09
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XIA Fei1,2,LIANG Li-guo2,XIE Jun2(1.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China;2.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China);Isolation,Identification and Drug Susceptibility of a Pathogenic Bacterial Strain from Bluntnose Black Bream Megalobrama amblycephala[J];水产科学;2012-10
2 ZHANG Mei-chao1,CAO Ya-nan1,YAO Bin1,BAI Dong-qing2,ZHOU Zhi-gang1(1.Key Laboratory for Feed Biotechnology of the Ministry of Agriculture,Feed Research Institute, Chinese Academy of Agricultural Sciences,Beijing 100081,China; 2.Department of Fisheries Science,Tianjin Agricultural College,Tianjin 300384,China);Characteristics of quenching enzyme AiiO-AIO6 and its effect on Aeromonas hydrophila virulence factors expression[J];水产学报;2011-11
3 MU Wei,GUAN Ling-yu,WANG Qi-yao,LIU Qin,ZHANG Yuan-xing(State Key Lab.of Bioreactor Engin.,E.China Uni.of Sci.& Technol.,Shanghai 200237);Establishment of Edwardsiella tarda Ghosts System[J];微生物学杂志;2011-03
4 MING Jianhua1,2,XIE Jun2,XU Pao2,LIU Wenbin3,ZHOU Qunlan2,LIU Bo2,XI Bingwen2 1.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China; 2.Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes,Ministry of Agriculture;Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China; 3.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;Effects of emodin,vitamin C and their combination on biochemical parameters and two HSP70s mRNA expression of Wuchang bream (Megalobrama amblycephala) infected with Aeromonas hydrophila[J];中国水产科学;2011-03
5 WANG Ruixuan1,FENG Juan1,SU Youlu1,LAN Xiangbin2,WANG Jiangyong1(1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;2.American Soybean Association Shanghai Office,Shanghai 200336);Isolation of Photobacterium damselae subsp.piscicida from diseased golden pompano(Trachinotus ovatus Linnaeus) and its drug sensitivity[J];中国水产科学;2010-05
6 XU Hai-dong1,2,OU You-jun1,GUO Zhi-xun1,SU You-lu1,WANG Rui-xuan1 (1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China; 2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China);The pathogenicity of nervous necrosis virus to Trachinotus ovatus and sequence analysis of the coat protein gene[J];上海海洋大学学报;2010-04
7 TIAN Tian1 HU Huo-geng1,2 CHEN Chang-fu1 1.College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;2.Jiangxi Aquaculture Technology Promotion Stations,Nanchang 330046,China;Identification and Pathogenicity of Bacterial Pathogens Isolated in an Outbreak of Bacterial Disease of Bluntnose Black Bream,Megalobrama amblycephala[J];华中农业大学学报;2010-03
8 GUO Song-lin1,2,GUAN Rui-zhang1,2,FENG Jian-jun1,2,CHEN Chuan-qing1(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology forAquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China);Influences of Blood and Biochemical Parameters on American Eels (Anguilla rostrata) Challenged by Aeromonas hydrophila[J];集美大学学报(自然科学版);2010-02
9 XU Xiaojuan1,2,LI Jia'er1,OU Youjun1(1. South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;2. College of Fisheries and Life,Shanghai Ocean University,Shanghai 201306,China);Effects of salinity on embryonic development and early larvae in ovate pompano Trachinotus ovatus[J];南方水产;2009-06
10 YU Lu-jun1,2,LI Ning-qiu1,LIU Li-hui1,FU Xiao-zhe1,SHI Cun-bin1,PAN Hou-jun1,WU Shu-qin1(1.Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China;2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China);Isolation and identification of pathogenic bacteria from Anguilla janponica mixedly infected with Edwardsiella tarda and Vibrio vulnificus[J];中国人兽共患病学报;2009-08
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