Study on the correlation between CGG triplet repeat ploymorphism of VLDL-R gene and atherosclerosis brain infarction.
Shao Hua 1, Zhou Xin, Tian Song 1 et al. 1Department of Clinical Laboratory, People′s Hospital of Wuhan University, Wuhan 430071
Objective To explore the correlation between CGG triplet repeat polymorphism on the 5′ non coding region of very low density lipoprotein receptor (VLDL R) gene and atherosclerosis brain infarction (ABI) in Chinese Han nationality. 225 subjects with no blood links in Hubei region, including 65 cases of ABI and 160 healthy controls were studied. Methods Polymerase chain reaction (PCR) denature polyacrylamide gel electrophoresis silver stain technique was employed to determine the CGG triplet repeat polymorphism of VLDL R gene and its relation with blood lipid level.Results The Chinese Han nationality in Hubei region had VLDL R gene polymorphism, 4 alleles with 5, 8, 9, 11CGG triplet repeats and 8 genotypes with 5/5, 5/8, 8/8, 8/9, 9/9, 5/9,8/11 and 5/11 were detected. There were no significant differences ( P 0.05 ) in VLDL R gene allele and genotype frequencies between ABI and normal controls. ABI groups had abnormal level of blood lipid. When the subjects were divided into 5 groups according to VLDL R genotype, the differences among the subgroups were not statistically significant.Conclusion There was VLDL R gene polymorphism in the Han Chinese in Hubei region. CGG triplet repeat polymorphism of VLDL R gene had no correlation with ABI. It is not an independent risk factor. VLDL R gene polymorphism does not have obvious influence on lipid metabolism.
【CateGory Index】： R743.33