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Effects of electromagnetic fields on differentiations of rat BMSCs under high glucose environment

LIU Yang,YANG Yong,YU Ji-zhe,et al.Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China  
Objective To investigate the effects of the sinusoidal electromagnetic fields(EMF) on the differentiation of rat bone marrow mesenchymal stem cells(BMSCs) under the high glucose environment,and discuss the potential molecular mechanism.Methods BMSCs were isolated by using adherence screening method,and divided into four groups: EMF group(pulsed sinusoidal EMF of 1 mT,and 50 Hz for 2 h per day),high glucose group(15 mmol/L),EMF with high glucose group,and control group.Alizarin red staining and oil red staining were used to observe the formation of calcium nodules and lipid droplets.Real-time PCR was used to evaluate the expression of osteogenic/adipogenic genes Rux2,ALP,Id4,PPARγ,and aP2.Results The ability of calcium nodule formation was enhanced and osteogenic genes were highly expressed in EMF group as compared with control group.The ability of lipid droplet formation was enhanced and adipogenesis-relative genes were highly expressed in high glucose group.Meanwhile,the osteogenic and adipogenic differentiation was both induced in EMF with high glucose group,but the former was more obvious.Conclusion Sinusoidal EMF promote BMSCs osteogenic differentiation and inhibit adipogenic differentiation indirectly under high glucose environment,which is probably related to the up-regulation of the differentiation key factor Id4.
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