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Effect of uniaxial cyclic tensile stretch on the proliferation of rat precartilaginous stem cells

YANG Kai-xiang,WU Ying-xing,DU Yu,et al.Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China.  
Objective To investigate the proliferation of the rat precartilaginous stem cells(PSCs) in response to different uniaxial cyclic tensile stretch.Methods The rat PSCs were isolated and purified using the magnetic activated cell sorting and cultured in vivo.The PSCs at third generation were stimulated by a four-point bending system at different tensile stretch(2 000,4 000 μstrain) and different time points(1,2,4 and 8 h).Flow cytometry was used to explore the changes of cell cycle and proliferation index(PI).Results Tensile stretch could influence the proliferation of PSCs.As compared with the control group,the 2 000-μstrain mechanical stretch could promote the proliferation of PSCs,with increased PI(P0.05) and the synthesis ratio of DNA(P0.05) in S phase.But 4 000-μstrain mechanical stretch could inhibit the proliferation of PSCs,with decreased PI(P0.05) and the synthesis ratio of DNA(P0.05) in S phase.Conclusion Proper tensile stretch could facilitate the proliferation of PSCs,while overloaded tensile stretch could inhibit it.
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