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《》 1985-01
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Zhou Jun chu  
Reproductive growth of bacteroids from protoplasts, as well as fromsuspsions of crushed nodules of soybean, was regularly observed directly inmicrochamber and determined as colony-forming ability (CFA) on labora-ory media. 1. Immaturc bacteroids (2 weeks after inoculation) with very low CFAare long thin rods, mature (5-week old nodule) with changeable CFA aresimilar to cultured brady-rhizobia. 2. Bacteroids from mature nodules were able to reproduce, their CFAincreased with the age of nodule and conditions of isolation. 3. The favourable conditions for bacteroid growth are distilled water orPDB-(as diluting solution), BMM(as medium) and pH 5.0-7.0. 4. A heat-hbile extract from soybean nodules and roots-increased therecovery of bacteroids marketly. Yeast extract and 5 vitamins did not showthis effect. 5. Osmoprotection(provided by the concentration of mannitol either 37g/lin BMM or 0.5M/l in PDB) Was not beneficial and even detrimental for thegrowth of bacteroids. 6. The growth from bacteroids was followed and recorded by microcham-ber incubation with video recording. Generation time of bacteroids was progre-ssively reduced and became identical with the cultured bradyrhizobium afterlst divition. 7. The identity of nodule bacteroids and isolates with the tested strainwas proved by fluorescent antibody technique.
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