TNE DUAL EFFECTS OF BR IN PROMOTING CALLUSE FORMATION AND DIFFERENTIATION IN BRASSICA NAPUS
Cai Detian, Chang Jinqiao, Zhu Hun, Luo Bingshan( Huazhong Agricultural University, Wuhan )
The effect of Brassinolide on rape tissue culture was studied. With the concentration ranging from 10~(-5) to 10~(-1) ppm Brassinolide ( BR ) promoted the calluse formation of rape hypocotyl. Proper concentration of BR also promoted rooting from the hypocotyl. The calluse formation was obviously stimulated by the combinations of BR with IAA, 2,4-D or GA_3 respectively. The calluse direved from the combination of BR and AA had higher differentiation frequency than that from BR and 2,4-D. And their pla(?)lets had good quality. It had important significance for some species in tissue culture,a BR promoted calluse formation and differentiation.