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《Journal of Huazhong Agricultural University》 2009-01
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Effects of Inhibin Gene Immunization on Reproductive Hormone Secretions in Nanyang Yellow Cattle

WANG Shui-lian1,2) XUE Li-qun2) CHEN Xiao-jun2) YANG Li-guo1)(1)College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2)College of Animal Medicine,Hunan Agricultural University,Changsha 410128,China)  
A total of 40 Nanyang Yellow cattle heifers were given double injections of either 3.0 mg inhibin eukaryotic expressing plasmid(pCIS,n=30 as an immunized group) or an equal volume of physiological saline(n=10 as a control group) at an interval of 21 days.Blood samples were collected on day 0,10,21 after the primary immunization,and on day 10 and 45 after the booster immunization.Plasma concentrations of follicle stimulating hormone(FSH),17 β-estradiol(E2) and progesterone(P4) were measured by a radioimmunoassay(RIA).The results showed that the highest percentage of the cattle with positive antibodies against inhibin was 37.5%,which was observed on the 10th day after booster immunization,the percentage of positive antibodies on day 10 was significantly different from those on the other days(P0.05).In addition,FSH level in the immunized group was a little higher than that in the control group(P0.05) with no significant difference;the difference was significant for both 17 β-estradiol(E2) and progesterone(P4) levels after the booster immunization of the tested group and the control.In addition,there is no difference for the mentioned three hormones in the positive antibody cattle in contrast to the negative antibody ones.All the results demonstrate that the recombined plasmid of inhibin gene can stimulate yellow cattle to produce antibodies against inhibin as well as have an effect on FSH,E2 and P4 content.
【Fund】: 国家自然科学基金项目(30070555);; 湖南农业大学人才稳定基金项目(07WD03);; 湖南农业大学动物医学院科研基金项目(SR0348)资助
【CateGory Index】: S823
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