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《Journal of Huazhong Agricultural University》 2013-01
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Genetic analysis of apple rootstock germplasms from Guizhou Province using ISSR markers

SONG Sha1,2 WU Ya-wei1 JI Xiang-biao3 SONG Lai-qing4 WEN Xiao-peng2 1.Institute of Fruit Tree of Guizhou Academy of Agricultural Sciences, Guizhou Province,Guiyang 550006,China; 2.Key Laboratory of Agricultural Bioengineering at Guizhou Province, Guizhou University,Guiyang 550025,China; 3.College of Life Science,Guizhou University,Guiyang 550025,China; 4.Institute of Fruit Tree in Yantai City Academy of Agricultural Sciences,Yantai 264000,China  
The genetic relationship of 15 apple rootstock germplasms were studied using ISSR markers.Ten pairs of primers were used and a total of 75 bands were yielded,among which 67 were polymorphic.Primers 827,M05 and M08 could be used for effectively genotyping the 15 germplasms.The systematical cluster analysis showed that 15 apple rootstock germplasms could be divided into three groups:Malus,Pyrus and Sorbus with a genetic similarity coefficient of 0.66,which fitted well with the morphological genealogy.
【Fund】: 贵州省农业科技攻关项目(黔科合字NY(2006)3003);; 贵州省科研机构创新能力建设项目(黔科合院所创能[2010]4009);; 贵州省农科院专项(黔农科院院专项[2010]044)
【CateGory Index】: S661.1
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