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《中国科学:生命科学(英文版)》 2018-01
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Atomic force microscopy studies on cellular elastic and viscoelastic properties

Mi Li;Lianqing Liu;Ning Xi;Yuechao Wang;State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences;Department of Industrial and Manufacturing Systems Engineering, The University of Hong Kong;  
In this work, a method based on atomic force microscopy (AFM) approach-reside-retract experiments was established to simultaneously quantify the elastic and viscoelastic properties of single cells. First, the elastic and viscoelastic properties of normal breast cells and cancerous breast cells were measured, showing significant differences in Young’s modulus and relaxation times between normal and cancerous breast cells. Remarkable differences in cellular topography between normal and cancerous breast cells were also revealed by AFM imaging. Next, the elastic and viscoelasitc properties of three other types of cell lines and primary normal B lymphocytes were measured; results demonstrated the potential of cellular viscoelastic properties in complementing cellular Young’s modulus for discerning different states of cells. This research provides a novel way to quantify the mechanical properties of cells by AFM, which allows investigation of the biomechanical behaviors of single cells from multiple aspects.
【Fund】: supported by the National Natural Science Foundation of China (61503372 61522312 U1613220 61327014 61433017);; the Youth Innovation Promotion Association CAS (2017243);; the CAS FEA International Partnership Program for Creative Research Teams
【CateGory Index】: R318.01
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