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《Basic & Clinical Medicine》 2009-07
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PP60C-SRC/ERK1/2 regulates the viability of rat spermatogonial stem cells

CHEN Jia-xiang1#,XU Si-fan2#,WANG Jing-lei1*,YANG Jun-ling1,QIN Hai-yan1,ZOU Ting1(1.Department of Physiology,Medical College of Nanchang University,Nanchang 330006;2.Life and EnvironmentalScience College,Central University for National Minorities,Beijing 100081,China)  
Objective To investigate the effect of PP60C-SRC on the viability of rat spermatogonial stem cells in 9 day-old rat.Methods MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense C-SRC oligodeoxynucleotides(ODNs) and PD98059 in vitro;Western blot was used to observe the PP60C-SRC and phosphorylated extracellular regulated kinase1/2(p-ERK1/2) contents.Results Compared with that in control group,the viability of the cells was decreased by 8.1%(P0.05) and 9.4%(P0.01),respectively,after treatment with antisense C-SRC ODNs and PD98059.Compared with the control group,PP60C-SRC and p-ERK1/2 contents were decreased by 33.8% and 38.4%(both P0.01),respectively,after transfection of antisense C-SRC ODNs.Compared with the control group,the p-ERK1/2 content was decreased by 34.5%(P0.01) in the spermatogonial stem cells after treatment with PD98059.Conclusion PP60C-SRC regulates the viability of rat spermatogonia stem cells through p-ERK1/2.
【Fund】: 国家自然科学基金(30360032);; 南昌大学(医科类)基金(NC2007004);; 江西省教育厅基金(GJJ09444)
【CateGory Index】: R363
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 ZHANG Wen,ZHANG Xuan*(Department of Rheumatology,PUMC Hospital,CAMS & PUMC,Beijing 100730,China);Role of TRAF2 in AP-1 signaling pathway of human B cells[J];Basic & Clinical Medicine;2008-05
2 YANG Jun-ling~1,XU Si-fan~(1*),CHEN Jia-xiang~1,YU Rong-jiao~1,ZENG Xin~1,WANG Jing-lei~1,KUANG Hai-bin~1(1.Department of Physiology,Medical School of Nanchang University,Nanchang 330006,China;2.Life and Environmental Science College,Central University for National Minorities,Beijing 100081,China);CULTURE IN VITRO OF TYPE A SPEMATOGONIAL CELLS[J];Acta Anatomica Sinica;2006-04
3 CHEN Jia-Xiang1, WANG Xin-Chang1, WANG Jing-Lei1,*, XU Si-Fan2, QIN Hai-Yan1, YANG Bei1, YANG Jun-Ling1, ZOU Ting1 1Department of Physiology, Medical College of Nanchang University, Nanchang 330006, China; 2Life and Environmental Science College, Central University for National Minorities, Beijing 100081, China;Proto-oncogene c-src regulates the viability of rat spermatogonial stem cells in vitro through phosphorylated signal transducer and activator of transcription-3[J];Acta Physiologica Sinica;2008-03
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Wen,ZHANG Xuan*,ZENG Xiao-feng,ZHANG Feng-chun(Department of Rheumatology,PUMC Hospital,CAMS & PUMC,Beijing 100730,China);Role of TRAF6 in NF-κB and AP-1 signaling transduction pathway in human B lymphoma cell line[J];Basic & Clinical Medicine;2009-07
2 YAN Deng-ke~1,XU Si-fan~2,WANG Jin-lei~1,CHEN Jia-xiang~1(1.Medical College,Nanchang University Nanchang 330006,China;2.College of Life and Environmental Sciences,Central University for Nationalities,Beijing 100081,China);The Expression and Effect of Proto-oncogene c-raf in Spermatogonial Stem Cells[J];Journal of the Central University for Nationalities(Natural Sciences Edition);2007-03
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