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《Journal of Jilin Agricultural Sciences》 2014-01
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Genetic Transformation of Na~+ Transporter Gene SKC1 into Soybean Mediated with Agrobacterium

YU Zhi-jing;CAI Qin-an;LIU Yan-zhi;QI Guang-xun;MA Rui;DONG Ying-shan;Institute of Agricultural Bio-Technology Research, Jilin Academy of Agricultural Sciences;  
In this study the plant expression vector pTF-SKC1, harboring a Na+ transporter gene SKC1 which is derived from rice and a selection marker gene bar, was constructed. The gene was transformed into soybean mediated with Agrobacterium tumefaciens by using cotyledon node as explants. PPT resistant regenerated plants were identified by PCR, and the rate of positive plants was 50%. The test for salt-tolerance of the transformed plants showed that over-expressing of SKC1 in soybean could enhance salt tolerance of the transgenic plant of soybean.
【Fund】: 转基因生物新品种培育重大专项(2011ZX08004-002)
【CateGory Index】: S565.1
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XUE Ren-gao (College of Life Science,Qingdao Agricultural University,Qingdao,Shandong 266109);Study on the Factors Affecting the Genetic Transformation Efficiency of Soybean Germinating Seeds Mediated by Agrobacterium tumefacines[J];Journal of Anhui Agricultural Sciences;2008-07
2 LI Tao et al(Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences,Branch of National Center for Soybean Improvement,Key Laboratory of Crop Genetics and Breeding,Shijiazhuang,Hebei 050031);Research Progress on Transgenic Technology of Soybean[J];Journal of Anhui Agricultural Sciences;2009-22
3 ZHOU Si jun 1,LI Xi chen 1,LIU Zhao jun 1, LIU Li yan 1 and YANG Qing kai 2 (1 Biotechnology Research Center,Heilongjiang Academy of Agricultural Sciences,Harbin 150086; 2 Institute of Soybean,Northeast Agricultural University,Harbin 150030;Optimization of the Agrobacterium-mediated transformation systems of soybean[J];Journal of Northeast Agricultural University;2001-04
4 FENG Tao1,SUN Congshu1,LIU Lijun1,ZU Wei1,HAN Tianfu2(1.College of Agriculture,Northeast Agricultural University,Harbin 150030,China; 2.Chinese Academy of Agricultural Sciences,Beijing 100081,China);Advances in the genetic transformation of soybean and its application in agriculture[J];Journal of Northeast Agricultural University;2008-03
5 WU Xiaoxia1,LI Jing1,LIU Weiting2,LIU Shanshan1,WANG Zhikun1,LI Wenbin1 (1.Key Laboratory of Soybean Biology,Ministry of Education,College of Agriculture,Northeast Agricultural University,Harbin 150030,China;2.Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China);Optimization study on strain cultivation period and infectious concentration on soybean cotyledonary node via Agrobacterium-mediated transformation system[J];Journal of Northeast Agricultural University;2010-01
6 WU Xiaoxia1,LI Jing1,WANG Zhikun1,LIU Shanshan1,LI Haiyan1,MA Yong2,LI Wenbin1(1.College of Agriculture,Northeast Agricultural University,Chinese Education Ministry's and Provincial Key Laboratory of Soybean Biology,Harbin 150030,China;2.Qitaihe Government Agricultural Service Center of Xinxing District Changxing Township,Qitaihe Heilongjiang 154532,China);Effect of AS concentration and pH on soybean genetic transformation[J];Journal of Northeast Agricultural University;2010-05
7 WU Xiaoxia 1,LIU Weiting 2,LI Jing 1,LIU Shanshan 1,WANG Zhikun 1,and LI Wenbin 1 1 College of Agriculture,Northeast Agricultural University,Chinese Education Ministry and Provincial Key Laboratory of Soybean Biology,Harbin 150030,China 2 Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Strain Cultivation Stage and Infectious Concentration on Soybean Cotyledonary Node via Agrobacterium–mediated Transformation System[J];东北农业大学学报(英文版);2010-02
8 Zhou Sijun 1 Li Xichen 1 Liu Zhaojun 1 Liu Liyan 1 Yang Qingkai 2 (1 Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences, Harbin 150086; 2 Institute of Soybean, Northeast Agricultural University, Harbin 150030);INTRODUCTION OF Bt GENE(cryIA)INTO SOYBEAN BY Agrobacterium-MEDIATED TRANSFORMTION[J];Soybean Science;2001-03
9 Li Haiyan Zhu Yanming ** Feng Yingying Zhou Changmei Liu Beidong Ding Xiaodng Zhang Shuzhen (Department op Plant Bioengineering,College of Life Sciences Northeast Agricultural University, Harbin 150030);ESTABLISHMENT OF EMBRYOGENIC SUSPENSION WITH IMMATURE COTYLEDONS OF SOYBEAN (Glycine max(L.)Merr.) AND INDUCTION OF SECONDARY EMBRYOS[J];Soybean Science;2002-02
10 Wang Xiaochun 1 Liu Shangqian 1 Ji Jing 2 Wang Ping 2 (1 Department of Agronomy,Hebei North University, Xuanhua 075131; 2 Changchun University of Agricultural and Animal Science,Changchun 100062);THE AFFECT FACTORS ON THE GERMINATION FREQUENCY OF SOMATIC EMBRYOS OF SOYBEAN[J];Soybean Science;2004-02
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