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《Journal of Jilin Agricultural Sciences》 2014-01
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Studies on Biochemical Characteristics of Isoenzymes of Amylase in Wheat Seeds

LI Yan-hong;CHENG Xiao-yan;HAN Zhuo;SUN Han-ju;College of Biology and Food Engineering, Hefei University of Technology;  
The biochemical characteristics of isoenzymes of the amylase in three major parts i.e. embryo, endosperm and radicle of wheat seeds of during germination were investigated by the native PAGE. The results showed that the optimum pH value was approximately 5.2 and the enzymatic activity was relatively stable during pH 5.0~9.0. The optimum temperature was 40℃ while most of the amylase isoenzymes kept their activities during 0℃~50℃. Heavy metal Hg2+showed the most serious inhibition on the activities of isoenzymes of amylase and their activities were almost totally inhibited. Other metal ions such as Cu2+, Pb2+, Zn2+, and Ca2+also showed inhibition on the activities of isoenzymes of amylase to different extent.
【Fund】: 2010年度安徽省长三角科技联合攻关项目(101407 02023);; 2012-2013学年合肥工业合肥工业大学2012年校级大学生创新训练计划项目(2012CXCY394);; 2011年国家自然科学基金项目(31171787);; 2010年安徽高校省级科学研究项目(010AJZR0899)
【CateGory Index】: S512.1
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