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《Journal of Jilin Agricultural Sciences》 2014-05
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Studies on Factors Influencing Embryogenesis of Isolated Microspores of Chinese Cabbage

CHEN Xiao-feng;Zhang Ming-pei;Xu Rong-xue;SUI Hao-lin;Yantai Research Institute of China Agricultural University;Seed Station of Longkou City;  
Factors affected culture of microspore was studied on four Chinese cabbage varieties of 'Fushan-baotou'. Results showed that there was significant difference in embryogenesis ability among different genotypes.The yield of microspore-derived embryo ranged from 1.28 to 38.25. The yield of microspore-derived embryo reach the highest level when the length of flower buds was from 2.1 mm to 3.0 mm, and extremely low when the length of flower buds was more than 3.0 mm or less than 2.0 mm. NLN-13 was the best medium for microspore culture, and hormone added did not improve microspore-derived embryo yield, but addition of 0.1 g/L to 0.3 g/L activated charcoal obviously increased microspore embryo production.
【Fund】: 中国农业大学烟台研究院课题(YT200907);; 烟台市科学技术发展计划项目(2010248)
【CateGory Index】: S634.1
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