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《Journal of Jilin Agricultural University》 2004-01
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Changes of Activities of Antioxidant Enzymes During Maize Seed Germination

WU Xian-jin~(1,2), SONG Song-quan~2, TIAN Xiang-rong~2, LEI Yan-bao~2, FU Jia-rui~2 (1. Department of Bioengineering, Huaihua College, Huaihua, Hu′nan 418008, China; 2. School of Life Sciences,Zhongshan University, Guangzhou, Guangdong 510275,China)  
Maize (Zea mays L.) seeds were used as experimental materials. MDA content and activity of antioxidant enzymes (SOD,POD,CAT) in embryos during seed germination were studied. The results showed that at 25℃, seeds began to sprout after being imbibed 16 h; the time needed for the germination of 50% was 38 h,and 100% was 66 h. There was some activity of SOD when the seeds were imbibed (0 h). The activity of SOD increased during the early periods of germination, but it decreased at the late periods of germination. It increased quickly before a few seeds germinated (imbibed 24 h),then it increased slowly when imbibed from 24 h to 35 h. When the germination percentage reached 70% (imbibied (42 h)), the activity of SOD initiated to decrease. There was very low POD activity when the seeds were imbibied 0 h . It increased gradually during the seed imbibition. The speed of POD activity increased with the quickening of imbibition. The activity of CAT was zero when the seeds were imbibied (0 h). It increased during seed (germination,) but when seed germination percentage reached 90%, it initiated to decrease. The changing tendency of MDA content during seed imbibition was decreasing, increasing and then decreasing again.
【Fund】: 湖南省教育厅资助项目(02C296);; 国际植物遗传资源研究所(IPGRI 00 076)资助项目
【CateGory Index】: S513
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