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《Journal of Jilin Agricultural University》 2004-01
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Study on Gene Cloning and mRNA Distribution of Pig Ghrelin

YANG Lian-yu~(1,2), YANG Wen-yan~2, JI Feng-jie~2, ZHAO Ying-cai~2, JIA Rui~1, WANG Zhe~1 (1. Department of Military Veterinary, the Quartermaster University of PLA, Changchun, Jilin 130062, China; 2. College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China)  
Total RNA was extracted from tissue of pig. 282 bp fragment was obtained by RT-PCR, the primer of which had been devised according to the published pig Ghrelin mRNA sequence. After being cloned in pMD-18T, the PCR segment was determined as Ghrelin cDNA. The results showed the distribution of Ghrelin mRNA in hypothalami, stomach, duodenum, jejunum, ileum, liver, kidney, heart and pancreas in 90 d pig and postnatal pig.
【Fund】: 国家自然科学基金资助项目(30170713)
【CateGory Index】: S828
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【References】
Chinese Journal Full-text Database 9 Hits
1 JIA Cui-Ping LEI Zhi-Hai NING Hong-Mei SU Juan ZHANG Wen-Long JIA Xiao-Qing(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China);Immunohistochemical Localization of Ghrelin-containing Cells in the Chinchilla Rabbit[J];Chinese Journal of Zoology;2007-04
2 DUAN Xiao-ming, FAN Jin-xiu, GUO Rui, LI Jia-kui(College of Veterinary Medicine,Huazhong Agricultural University, Wuhan 430070,China);Construction of pGherlin Eukaryotic Expression Plasmid and its Effect on the Rat Growing[J];China Animal Husbandry & Veterinary Medicine;2008-09
3 WANG Yue YU Shi-Yuan~ GAO Xian-Jun WANG Yu GUO Ting-Ting(College of Life Science,Northwest Normal University,Lanzhou 730070,China);Expression of Ghrelin,KGF and TGF-β (β_2,β_3) in Gastrointestinal Tract of Buteo hemilasius[J];Chinese Journal of Zoology;2009-06
4 ZHENG Xin~(1,2),LIU Guo-wen~(1,3),LIANG Hai-tao~2,XIA Qing-juan~2,HAO De-wei~2,WANG Zhe~1(1.College of Animal Husbandry and Veterinary,Jilin University,Changchun 130062,China;2.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;3.College of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao 028000,China);Effect of Copper on the Insulin-like Growth Factor and Binding Protein(s) in the Porcine Culture Hepatocytes[J];Journal of Jilin Agricultural University;2006-03
5 YANG Wen-yan~1,YANG Lian-yu~(2,3),GENG Chun-yin~3,ZHAO Ying-cai~3(1.College of Animal Husbandry and Veterinary Science,Jilin University,Changchun 130062,China;2.College of Environmental and Biological Science,Dalian University of Technology,Dalian 116000,China;3.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China);Construction of Eukaryotic Expression Recombinant Plasmid of Swine Ghrelin Gene and Histogenic Immunity Examination in Mice[J];Journal of Jilin Agricultural University;2006-06
6 Ailing Zhang1,2,Li Zhang1,3,Hong Chen1,4,Liangzhi Zhang1,Xianyong Lan1,Chunlei Zhang4,Cunfang Zhang1,and Zeyi Zhu11 College of Animal Science and Technology,Laboratory of Molecular Biology for Agriculture,Northwest A & F University,Yangling 712100,China 2 College of Basic Medical Sciences,Anhui Medical University,Hefei 230032,China 3 Agricultural College,Guangdong Ocean University,Zhanjiang 524000,China 4 Institute of Cellular and Molecular Biology,Xuzhou Normal University,Xuzhou 221000,China 5 Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850,China;Cloning,prokaryotic expression of cattle Ghrelin gene and biological activity detection of the expressed protein[J];Chinese Journal of Biotechnology;2009-01
7 GUO Ya-fen,LUO Qin,WANG Shi-kai,LAN Gan-qiu,WANG Ai-de(College of Animal Science and Technology,GuangXi University,Nanning 530004);Cloning and Sequence Analysis of Ghrelin cDNA from Guangxi Bama Minipig[J];Laboratory Animal Science;2007-06
8 ZHU Wen-qi,LI Hui-fang,SONG Wei-tao,XU Wen-juan,WANG Qiang,CHEN Kuan-wei(Jiangsu Institute of Poultry Science,Provincial key lab.of PoultryGenetics and Breeding,Yangzhou 225003,Jiangsu,China);The Genetic Effect of Ghrelin Gene on Egg Performance in Gaoyou Duck[J];Journal of Sichuan Agricultural University;2009-03
9 YANG Lian-yu~(1,2),YANG Wen-yan~1,ZHAO Ying-cai~(2),QIAN Jian~3,WANG Zhe~(1*) (1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;3.College of Animal Science and Technology,Tarim University of Agricultural Reclamation,Alar,Xingjiang 830000,China);Chemical Synthesis and Prokaryotic Expression of Ghrelin of Pig[J];Chinese Journal of Veterinary;2005-06
【Citations】
Chinese Journal Full-text Database 1 Hits
1 YANG Lian-yu, YANG Wen-yan, QIAN Jian, WANG Zhe ( Faculty of the Military Veterinary, Quartermaster University of PLA , Changchun , Jilin 130062, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118 China ; Institute of Animal Science and Technology, Tarim University of Agricultural Reclamation , Alar Xinjiang, 830000, China);Primary Attempt of Mixed Hypothalamus Nerve Cell Culture[J];Journal of Jilin Agricultural University;2003-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Liang Yu;Tuab Shufang;Liu Wenjun;LiJun;Ruan Li;Zhu Jimin(C.M.Chu)(Institute of Virology,CAPM,Beijing 100052)Eight plasmid vectors expressing HBsAg in mammalian cells were constructed,inwhich the sequence of receptor-binding site(21-47aa)for;NUCLEIC ACID VACCINATION IN MICE CAN INDUCEIMMUNE RESPONSES TO BOTH S AND PreS1(21-47aa)REGIONS of HBsAg[J];Chinese Journal of Virology;1996-03
2 CHEN Yu-Qin,YU Shi-Yuan,ZHANG Hu-Lin,ZHANG Jing,SHANG Jian-Ke,XIE Ming-Ren College of Life Science,Northwest Normal University,Lanzhou 730070,China;Histological structure and expression of EGFR,TGF-β,AQP-2 in Chrysolophus pictus kidney[J];Acta Zoologica Sinica;2008-02
3 HUANG Xi Gui JIAO Bao Wei WAND De Shou (Faculty of Life Science,Southwest China Normal University,Key Lab of Aquaculture Technology, Chongqing 400715,China);GHS-R: A Novelly Identified GH-secreting Protein[J];Chinese Journal of Zoology;2004-02
4 NIU Hong-Xing BU Yan-Zhen WANG Yan-Mei JI Sheng-DongYU Yan LU Quan-Wei XU Cun-Shuan(College of Life Sciences,Henan Normal University,Xinxiang 453007,China);Analysis of Proteolytic Enzymes in Digestive System of Buteo hemilasius[J];Chinese Journal of Zoology;2006-03
5 JIA Cui-Ping LEI Zhi-Hai NING Hong-Mei SU Juan ZHANG Wen-Long JIA Xiao-Qing(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China);Immunohistochemical Localization of Ghrelin-containing Cells in the Chinchilla Rabbit[J];Chinese Journal of Zoology;2007-04
6 ZHANG Yu-fang, WANG Hai-ning , HONG Tian-pei Department of Endocrinology, Peking University Third Hospital, Beijing 100083;Ghrelin expression in the tissues of different thyroid diseases[J];Journal of Peking University(Health Sciences);2006-02
7 LI Lai xing,YI Xian feng,LI Ming cai,ZHANG Xiao ai (Northwest Institute of Plateau Biology,the Chinsese Academy of Sciences,Xining,Qinghai 810001,China);Diet Composition of Upland Buzzard:Alnalysis on Stomach Content and Food Pellet[J];Zoological Research;2004-02
8 SONG Qin-ye~(1,2),YANG Han-chun~2 (1.Agricultural School, Hebei Engineering College,Handan,Hebei,057150,China; 2.College of Veterinary Medicine, China Agricultural University, Beijing,100094,China);The Research Advance on DNA Vaccine[J];Progress In Veterinary Medicine;2004-03
9 JIANG Qing-dong 1 ,WANG Yan-ling 2 ,WANG Yue-ying 1 (1.College of Animal Husbandry and Veterinary Engineering,Henan Agricultural University,Zhengzhou,Henan,450002,China; 2.College of Animal Science and Technology,Northwest A & F University,Yangling,Shaanxi,712100,China);Progress on Somatostatin and Its Receptor[J];Progress In Veterinary Medicine;2005-06
10 WEI Tai yun, LIN Han xin, XIE Lian hui (Institute of Plant Virology, Fujian Agriculture and Forestry University,Fuzhou, Fujian 350002, China);Optimization of the conditions affecting PCR-SSCP analysis[J];Journal of Fujian Agricultural University(Natural Science);2002-01
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 WANG Yue YU Shi-Yuan~ GAO Xian-Jun WANG Yu GUO Ting-Ting(College of Life Science,Northwest Normal University,Lanzhou 730070,China);Expression of Ghrelin,KGF and TGF-β (β_2,β_3) in Gastrointestinal Tract of Buteo hemilasius[J];Chinese Journal of Zoology;2009-06
2 YANG Wen-yan~1,YANG Lian-yu~(2,3),GENG Chun-yin~3,ZHAO Ying-cai~3(1.College of Animal Husbandry and Veterinary Science,Jilin University,Changchun 130062,China;2.College of Environmental and Biological Science,Dalian University of Technology,Dalian 116000,China;3.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China);Construction of Eukaryotic Expression Recombinant Plasmid of Swine Ghrelin Gene and Histogenic Immunity Examination in Mice[J];Journal of Jilin Agricultural University;2006-06
3 Ailing Zhang1,2,Li Zhang1,3,Hong Chen1,4,Liangzhi Zhang1,Xianyong Lan1,Chunlei Zhang4,Cunfang Zhang1,and Zeyi Zhu11 College of Animal Science and Technology,Laboratory of Molecular Biology for Agriculture,Northwest A & F University,Yangling 712100,China 2 College of Basic Medical Sciences,Anhui Medical University,Hefei 230032,China 3 Agricultural College,Guangdong Ocean University,Zhanjiang 524000,China 4 Institute of Cellular and Molecular Biology,Xuzhou Normal University,Xuzhou 221000,China 5 Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850,China;Cloning,prokaryotic expression of cattle Ghrelin gene and biological activity detection of the expressed protein[J];Chinese Journal of Biotechnology;2009-01
4 NONG Wei,XIE Ti-san,ZHANG Xin-ming,WANG Xiao-li,LIU Qing-you,SHI De-shun(Animal Reproduction Institute,Guangxi University,Nanning 530005,China);Cloning and sequence analysis of Ghrelin gene in swamp buffalo[J];Animal Husbandry & Veterinary Medicine;2009-01
5 YANG Lian-yu1,2,YANG Wen-yan1,GENG Chun-yin3,LI De-li1,WANG Shu-jie1,QIN Gui-xin1*(1.Faculty of Animal Science and Technology,Jilin Agricultural University,Changchun 130118 China;2.Dalian Post Doctoral Research Center of SEM BIO-Engineering Technology Co.Ltd,Dalian,Liaoning 116023,China;3.College of Agriculture Science,Yanbian University,Longjing,Jilin 133400,China);Effects of PCI-Ghrelin-28aa gene plasmid on the performance of weaned piglets[J];Chinese Journal of Veterinary Science;2009-06
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 YANG Hao, LIANG Zhe, JU Gong  (Institute of Neurosciences,the Fourth Military Medical University,Xi'an\ 710032,China) \;;THE ONTOGENCY OF THE SRIF NEURONS IN THE HYPOTHALAMUS OF EMBRYONIC MICE in vitro──A STUDY OF IMMUNOCYTOCHEMICAL STAINING[J];ACTA ANATOMICA SINICA;2000-03
2 Pang Jijie;Mei Jun(Department of Brain and Nerve,Xi′an Medical University, Xi′an 710061);The aging change of the growth velocity of the neurites of the hypothalamic neurons in culture[J];CHINESE JOURNAL OF CERIATRICS;1995-02
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