Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Jilin Agricultural University》 2004-01
Add to Favorite Get Latest Update

Establishing Method of RT-PCR Detecting H_5,H_9 Subtypes of Avian Influenza Virus

LIU Yan, QIAN Ai-dong~() (College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118 , China)  
Two specific primers were designed according to the HA sequences reported and registered in Genebank of different strains of H_5,H_9 subtypes.The procedure using RT-PCR to detect H_5,H_9 subtypes of AIV was established and the optimal conditions of PCR for each subtype were determined by orthongonal assay. The method is very practable and specific. Its allergy is 10~(-3). It could be used in diagnosis of H_5,H_9 subtypes of AIV.The method was used to detect H_5 and H_9 subtypes of AIV, which hide in poultry and in the products of poultry . The rudimentary application of the RT-PCR has the same results as seperate cultivation of the virus.The positive results acquired were 30%higher than AG and EM.
【Fund】: 吉林省科技厅资助项目(20005514);; 长春市科技局资助项目(20099)
【CateGory Index】: S852.65
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 WANG Kun1,2,ZHANG Yu-jing1,SUN Zhao-zeng1,YAN Ruiqing1,ZHANG Lei1(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.Changchun Drug Inspection Institute,Changchun 130062,China);Cloning Expression and Antibody Preparation of Fused Gene NS1A of H9N2 Subtype Avian Influenza Virus[J];Journal of Jilin Agricultural University;2007-03
2 SUN Yan-yong1,LIU Zhi-jun1,WANG Ze2,LIAN Hai3,GAO Feng1,XIA Zhi-ping1,31.College of Animal Science and Veterinary Medicine,Jilin Univerity,Changchun 130062,China; 2.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;3.Institue of Veterinary Medicine,Academy of Military Medical Sciences of PLA,Changchun 130062,China;Diagnosis of Co-Infection of Porcine Reproductive and Respiratory Syndrome and Porcine Circovirus Type 2[J];Journal of Jilin Agricultural University;2009-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Rong-rong et al(Insitude of Animal Husbandry and Veterinary,Hubei Academy of Academy of Agricultural Science,Wuhan,Hubei 430064);Research Development of AIV Diagnostic Methods[J];Journal of Anhui Agricultural Sciences;2009-22
2 JIN Yuan chang,LI Jing peng,ZHANG Long,SUN Yue, YU Min,YANG Jia fang (Life Science College,Northeast Agricultural University,Harbin,Heilongjiang,150030,China);Progress on Avian Influenza Virus with Molecular Biology in China[J];Progress In Veterinary Medicine;2003-01
3 LI Zhi-xin~(1,2),ZHANG Zhi-qiang~(3),XIN Xiao-geng~(3),LIU Guang-yuan~(1)(1.Lanzhou Veterinary Research Institute,CAAS,Lanzhou,Gansu,730046,China;2.Faculty of Veterinary Medicine,Gansu Agricultrue University,Lanzhou,Gansu,730070,China;3.Technical Promotion Station of Animal Husbandry and Veterinary Medicine,Longde,Ningxia,756300,China);Development of the Diagnosis Methods of Avian Influenza[J];Progress in Veterinary Medicine;2006-S1
4 LI Liping~1,WEI Ping~1,WEI Tianchao~1,GAN Jiazhong~2,Chen Qing~2,LU Jing~2,FENG Chao~2(1 Institute for Poultry Science & Health,Guangxi University,Nanning 530005,China;2 Station of Husbandry and Veterinary Medicine,Yulin 537000,China);Molecular biological characteristics and diagnostic techniques of avian influenza virus——an update review[J];Journal of Guangxi Agricultural and Biological Science;2005-04
5 CHEN Si-huai1,2,QIAO Xian-feng3,HUA Wen-jun3,ZHENG Xin-ming3,WEI Qing-xin3,Zhang Yong1(1.Bio-engineer Institude,Northwest Sci-tech University of A&F,Yangling 712100,Shannxi,China; 2.Animal Sci-tech.College,Southwest University,Rongchang 402460,Chongqing,China; 3.Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agriculture Science,Wuhan 430064,China);Study on Rapid Detection of Avian Influenza Virus by Multi-RT-PCR[J];Hubei Agricultural Sciences;2006-01
6 LI Dong-ye,YU Li-yun;Minireview of Avian Influenza Research[J];Journal of Heilongjiang August First Land Reclamation University;2004-04
7 HOU Xi-lin,WANG Chun-ming,LI Qing-feng et al.;Epidemiology of Avian Influenza in Daqing Area of Heilongjiang Province[J];Journal of Heilongjiang August First Land Reclamation University;2005-06
8 HE Yue-ying,ZENG De-nian*,HU Shi-feng,NING Ling-zhong,YAN Mei-qing (College of Animal Science and Technology,HNAU,Changsha 410128,China);Morphologic Observation on Pathogen of Geese Virus Disease and Measurement on Its Physiochemical Properties[J];Journal of Hunan Agricultural University;2005-03
9 WANG Kun1,2,ZHANG Yu-jing1,SUN Zhao-zeng1,YAN Ruiqing1,ZHANG Lei1(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.Changchun Drug Inspection Institute,Changchun 130062,China);Cloning Expression and Antibody Preparation of Fused Gene NS1A of H9N2 Subtype Avian Influenza Virus[J];Journal of Jilin Agricultural University;2007-03
10 ZHANG Wen-hui1,WANG Wei-li2,ZHENG Cong1,LIU Ming3,QIAN Ai-dong1(1 College of Life Science,Jilin Agricultural University,Changchun 130118,China;2 Jilin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Changchun 130062,China;3 National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of CAAS,Harbin 150001,China);Study on Multiplex Reverse Transcription Polymerase Chain Reaction for Detection of H5,H7 and H9 Subtypes Avian Influenza Virus[J];Journal of South China Agricultural University;2009-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 DING Xiao-ran,YANG Jing,BO Xiao-chen,ZHANG Min-li,CHEN Su-hong,WANG Sheng-qi~*(Beijing Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China);The Inhibitive Effects of Combining an Antisense Oligodeoxynucleotides Targeted to ASGPR with Anti-HBV Drugs against Hepatitis B Virus in vitro[J];Chinese Journal of Virology;2006-01
2 QIN Zhi-feng~1,LV Jian-qiang~1,XIAO Xing-long~2,ZHONG An-qing~1,LIN Jin-zhong~2,HUANG Yun-sheng~1,LIN Qing-yan~1,CHENG Shu-kun~1,JIN Xian-zhong~1,LIN Miao~1,LIU Sheng-li~(1**)(1.Shenzhen Entry-Exit Inspection & Quarantine Bureau,Shenzhen 518045,China;2.Shenzhen Taitai Genomics Incorporation,Shenzhen 518057,China);Development of a Multiplex Real-time Reverse Transcriptase PCR Assay for Simultaneous Identification of Avian H5,H7 and H9 Hemagglutinin Subtypes[J];Chinese Journal of Virology;2006-02
3 TAO Yong-Guang, YUE Shao-Jie, YU Yan, et al. (Department of Pediatrics, Xiangya Hospital, Hunan Medical University, Changsha 4 10008, China);Effects of Qingkailing on Free Calcium of Synaptosomes in Rats with Neurotoxic Brain Edema Induced by L-glutamate[J];CHINESE JOURNAL OF CONTEMPORARY PEDIATRICS;2000-05
4 JIN Yuan chang,LI Jing peng,ZHANG Long,SUN Yue, YU Min,YANG Jia fang (Life Science College,Northeast Agricultural University,Harbin,Heilongjiang,150030,China);Progress on Avian Influenza Virus with Molecular Biology in China[J];Progress In Veterinary Medicine;2003-01
5 CHEN Hong-ying,LI Xin-sheng (College of Animal Science and Engineering,Henan Agriculture University,Zhengzhou,Henan,450002,China);The influenza virus Nucleoprotein:A Multifunctional RNA-binding Protein[J];Progress In Veterinary Medicine;2004-03
6 Xu Jianhua, Zhao Rong, Ke Danru, et al (Institute of Clinical Pharmacology, Fujian Medical University, Fuzhou, 350004);Effect of Curcumin on Cell kill Kinetics and Cell Cycle Phases of Human Hepatocarcinoma Bel7402 Cell[J];JOURNAL OF FUJIAN MEDICAL UNIVERSITY;1998-03
7 LI Liping~1,WEI Ping~1,WEI Tianchao~1,GAN Jiazhong~2,Chen Qing~2,LU Jing~2,FENG Chao~2(1 Institute for Poultry Science & Health,Guangxi University,Nanning 530005,China;2 Station of Husbandry and Veterinary Medicine,Yulin 537000,China);Molecular biological characteristics and diagnostic techniques of avian influenza virus——an update review[J];Journal of Guangxi Agricultural and Biological Science;2005-04
8 CHEN Si-huai1,2,QIAO Xian-feng3,HUA Wen-jun3,ZHENG Xin-ming3,WEI Qing-xin3,Zhang Yong1(1.Bio-engineer Institude,Northwest Sci-tech University of A&F,Yangling 712100,Shannxi,China; 2.Animal Sci-tech.College,Southwest University,Rongchang 402460,Chongqing,China; 3.Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agriculture Science,Wuhan 430064,China);Study on Rapid Detection of Avian Influenza Virus by Multi-RT-PCR[J];Hubei Agricultural Sciences;2006-01
9 WANG Chun-feng~1,YU Shou-ping~1,YANG Shu-bao~1,LI Yu~2(1.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;2.Institute of Mycology,Jilin Agricultural University,Changchun 130118,China);Cloning and Sequencing of Full Vp4 Gene of Group A Porcine Rotavirus[J];Journal of Jilin Agricultural University;2005-05
10 HE Hou-jun, DAI Yi-min, FU Guang-hua( College of Animal Science and Technology, JAU, Nanchang 330045, China );Progress in Molecular Biology of Avian Influenza Virus[J];Acta Agriculturae Universitis Jiangxiensis;2004-02
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 PENG Xiao-ling1,LAN Ling1,MA Ya-zhen1,CHENG Jin2(1.Institute of Animal Sanitary Supervision of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang,830063,China;2.Xinjiang Autonomous Region Academy of Animal Husbandry,Urumqi,Xinjiang,830000,China);Establishment of RT-PCR for Rapid Diagnosis of Highly Pathogenic avian influenza[J];Progress in Veterinary Medicine;2009-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved