Determination of low density lipoprotein subfraction particle size and its application
CHEN Da ning, WANG Jun jun, ZHANG Ling,ZHUANG Yi yi (Center of Medical Laboratory science,Jinling Hospital,Nanjing 210002,Chian)
Objecives: To determinate the size of low density lipoprotein (LDL) particle in patients with coronary artery diseases (CHD). Methods: Plasma LDL in 37 CHD patients and 26 control subjects were analyzed by 2%~16% non denaturing polyacrylamide gradient gels. Results: The diameters of LDL subfraction peaks were calculated by comparision with a standard calibration curve. LDL subfraction pattern was classified into pattern A (≥25.5 nm) and B (25.5 nm), according to diameter of major peak of LDL subfraction particle. The CHD patients had significantly less particle diameters than control subjects,(25.71±0.66) nm vs (26.41±0.95) nm, respectively( P 0.01). The distribution of LDL subfraction pattern was significantly difference between two groups ( P 0.05). Conclusions: Small density pattern B LDL subfraction may be a risk factor for atherosclerosis.