Experimental observations of intra-abdominal infection inducing multiple organ dysfunction syndrome
QUAN Zhu fu,WANG Yi jun,LIU Fang nan,SHI QUN li,LI Jie shou (Research Institute of General Surgery,Jinling Hospital,Nanjing 210002,China)
Objectives:The study was designed to observe the effect of intra abdominal infection(IAI) on the function and structure of organs and systems in rabbits. Methods:Twenty animals were randomly divided into two groups,one received cecal ligation plus puncture(CLP) inducing IAI and another received sham operation.Rectal temperature,blood gas,serum biochemical indexes,urine excretion of 3 methylhistidine (3 MH) were measured dynamically.The animals were maintained with infusion for one week and the death was recorded.Blood bacteria and pathological changes of the heart,lung,liver,kidney,small intestine and the spleen were examined at death or when killed. Results:Compared with control group,the temperature was increased in IAI group animals,the abnormalities of blood gas and serum biochemical indexes occurred,the urine excretion of 3 MH was risen,and the pathological changes of organs were remarkable.The incidence rate of bacteremia was 80% and the survival time was (84.1±39.0) hours in IAI group. Conclusions:The functional and pathological changes in organs and systems caused by CLP induced IAI are obvious and characteristic of multiple organ dysfunction syndrome.